Isolation of Monocytes from Bovine Blood for Purposes of Culture of Dendritic Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F19%3A43916589" target="_blank" >RIV/62156489:43210/19:43916589 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/19:N0000133

Výsledek na webu

<a href="http://dx.doi.org/10.15414/jmbfs.2019.9.special.427-430" target="_blank" >http://dx.doi.org/10.15414/jmbfs.2019.9.special.427-430</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15414/jmbfs.2019.9.special.427-430" target="_blank" >10.15414/jmbfs.2019.9.special.427-430</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Isolation of Monocytes from Bovine Blood for Purposes of Culture of Dendritic Cells

Popis výsledku v původním jazyce

Monocytes comprise a population of mononuclear leukocytes. In this article, we compare methods for isolating monocytes and also their cultivation into dendritic cells. Bovine blood collected from the vena jugularis externa was used for the experiment. This blood was used for isolating monocytes based on density gradient of OptiPrep or Histopaque solutions. The results indicated that the more efficient method is isolation based on density gradient of the Histopaque solution and subsequent magnetic separation of the cells. The monocyte population was further cultivated for 72 h with IL-4 and GM-CSF. The cells were evaluated under a light microscope, and it was apparent that CD14-positive cells transformed to dendritic cells. The second method using the Histopaque solution and magnetic separation was more efficient for the subsequent culture of dendritic cells.

Název v anglickém jazyce

Isolation of Monocytes from Bovine Blood for Purposes of Culture of Dendritic Cells

Popis výsledku anglicky

Monocytes comprise a population of mononuclear leukocytes. In this article, we compare methods for isolating monocytes and also their cultivation into dendritic cells. Bovine blood collected from the vena jugularis externa was used for the experiment. This blood was used for isolating monocytes based on density gradient of OptiPrep or Histopaque solutions. The results indicated that the more efficient method is isolation based on density gradient of the Histopaque solution and subsequent magnetic separation of the cells. The monocyte population was further cultivated for 72 h with IL-4 and GM-CSF. The cells were evaluated under a light microscope, and it was apparent that CD14-positive cells transformed to dendritic cells. The second method using the Histopaque solution and magnetic separation was more efficient for the subsequent culture of dendritic cells.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910212" target="_blank" >QK1910212: Moderní metody diagnostiky, terapie a prevence mastitid způsobených Streptococcus uberis jako nástroj pro sestavování cílených kontrolních programů v chovech skotu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiology, Biotechnology and Food Science

ISSN

1338-5178

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

Special Issue

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

4

Strana od-do

427-430

Kód UT WoS článku

000500544500014

EID výsledku v databázi Scopus

2-s2.0-85083482487