Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds

Popis výsledku v původním jazyce

This research was focused on investigation of gene expression of selected genes via the real-time quantitative PCR. Specific primers for CDK4 and TP53BP1 were designed on the basis of the sequences clones obtained from the subtractive hybridization and gene expression was analysed. Two groups of samples isolated skeletal muscle-specific RNA were compared, the first one was obtained from the Pietrain adult sows and their fetuses, the second one was obtained from Large White, respectively. The stability values of candidate reference genes (ACTB, EEF1A1, EEF2, GAPDH, H3F, HPRT1, PPIA and TOP2B) were made for both breeds. The expression levels of the investigated genes were varying by adult sows used as control samples. The higher or lower expression levelwas found out in few samples; one of the samples of gene CDK4 was expressed near to 0.001-fold, and two of samples of gene TP53BP1 were expressed higher then 18-fold, 52-fold respectively. The long-range goal of this research is to provi

Název v anglickém jazyce

Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds

Popis výsledku anglicky

This research was focused on investigation of gene expression of selected genes via the real-time quantitative PCR. Specific primers for CDK4 and TP53BP1 were designed on the basis of the sequences clones obtained from the subtractive hybridization and gene expression was analysed. Two groups of samples isolated skeletal muscle-specific RNA were compared, the first one was obtained from the Pietrain adult sows and their fetuses, the second one was obtained from Large White, respectively. The stability values of candidate reference genes (ACTB, EEF1A1, EEF2, GAPDH, H3F, HPRT1, PPIA and TOP2B) were made for both breeds. The expression levels of the investigated genes were varying by adult sows used as control samples. The higher or lower expression levelwas found out in few samples; one of the samples of gene CDK4 was expressed near to 0.001-fold, and two of samples of gene TP53BP1 were expressed higher then 18-fold, 52-fold respectively. The long-range goal of this research is to provi

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

VIIth International conference of PhD. and MSc. students "Genetics and Animal Breeding"

ISBN

978-80-7375-053-4

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

78-83

Název nakladatele

Ediční středisko MZLU v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

17. 5. 2007

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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