Employing of high performance liquid chromatography with UV-VIS and electrochemical detection for determination of phenols in plants tissues

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F08%3A00134102" target="_blank" >RIV/62156489:43210/08:00134102 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Employing of high performance liquid chromatography with UV-VIS and electrochemical detection for determination of phenols in plants tissues

Popis výsledku v původním jazyce

The optimized parameters were as follows: column and detector temperature 42 °C, flow rate of the mobile phase 0.75 ml/min. Under these parameters well developed and symmetric signals for the target molecules were measured. The detection limits (3 S/N) for naphthoquinones and phenols were estimated below 1 uM. In addition we quantified the secondary metabolites in plant tissues. The content of naphthoquinones was determined in Nepenthes. The methanolic extracts of Nepenthes tissues (12 hours long extraction) were analysed by optimized method and high content of plumbagin with low content of lawsone was determined. Further phenolic compounds were determined in tissues of fruits (hawthorn, bilberry, shadbush, honeysuckle). The totals of flavonoid peaks express the abundance of ROS-suppressing phenolic compounds (flavonoid compounds as a main antioxidant complex) contained in the samples of less common fruit species. Based on the results the antioxidant potential at various plant species

Název v anglickém jazyce

Employing of high performance liquid chromatography with UV-VIS and electrochemical detection for determination of phenols in plants tissues

Popis výsledku anglicky

The optimized parameters were as follows: column and detector temperature 42 °C, flow rate of the mobile phase 0.75 ml/min. Under these parameters well developed and symmetric signals for the target molecules were measured. The detection limits (3 S/N) for naphthoquinones and phenols were estimated below 1 uM. In addition we quantified the secondary metabolites in plant tissues. The content of naphthoquinones was determined in Nepenthes. The methanolic extracts of Nepenthes tissues (12 hours long extraction) were analysed by optimized method and high content of plumbagin with low content of lawsone was determined. Further phenolic compounds were determined in tissues of fruits (hawthorn, bilberry, shadbush, honeysuckle). The totals of flavonoid peaks express the abundance of ROS-suppressing phenolic compounds (flavonoid compounds as a main antioxidant complex) contained in the samples of less common fruit species. Based on the results the antioxidant potential at various plant species

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/EP0960006028" target="_blank" >EP0960006028: Zlepšení biologické kvality výsadbového materiálu s využitím netradičních postupů v ovocných školkách.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Vitamins 2008 - Nutrition and Diagnostics

ISBN

978-80-7318-708-8

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

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Název nakladatele

University of Pardubice

Místo vydání

Pardubice, Czech Republic

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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