Determination of GSH and GSSG in blood plasma of patiens by HPLC-ED

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F10%3A00184593" target="_blank" >RIV/62156489:43210/10:00184593 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of GSH and GSSG in blood plasma of patiens by HPLC-ED

Popis výsledku v původním jazyce

Thanks to many chemicals and physical stresses generated by outside environment there still is unpredictable danger of damaging of organism by reactive oxygen species (ROS). Biological harmfulness of ROS is generally given by the subsequent oxidation ofessential cellular structures. Organism is defending against ROS by synthesis of small molecules which can neutralize radicals. The most suitable existing organic molecule widespread in whole organism which can decrease effect of ROS is glutathione (GSH). Therefore we interested in changes of GSH levels by oncological patients. We optimized fast and robust method for analysis blood plasma of patients by employment of HPLC with coulometric detection. Our results based on hunderts of analyzed samples shows significant oxidation damage by disbalance between GSH/GSSG ratio.

Název v anglickém jazyce

Determination of GSH and GSSG in blood plasma of patiens by HPLC-ED

Popis výsledku anglicky

Thanks to many chemicals and physical stresses generated by outside environment there still is unpredictable danger of damaging of organism by reactive oxygen species (ROS). Biological harmfulness of ROS is generally given by the subsequent oxidation ofessential cellular structures. Organism is defending against ROS by synthesis of small molecules which can neutralize radicals. The most suitable existing organic molecule widespread in whole organism which can decrease effect of ROS is glutathione (GSH). Therefore we interested in changes of GSH levels by oncological patients. We optimized fast and robust method for analysis blood plasma of patients by employment of HPLC with coulometric detection. Our results based on hunderts of analyzed samples shows significant oxidation damage by disbalance between GSH/GSSG ratio.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biochemical Technology

ISSN

0974-2328

e-ISSN

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Svazek periodika

2

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

IN - Indická republika

Počet stran výsledku

2

Strana od-do

9-10

Kód UT WoS článku

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EID výsledku v databázi Scopus

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