THE USE OF MICROELUTION PLATE AS A PART OF HYPHENATED EXTRACTION METHOD FOLLOWED BY RRLC-MS-MS ANALYSIS FOR THE ISOLATION AND DETERMINATION OF PHENOLIC COMPOUNDS IN SEA ALGAE

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F10%3A00234796" target="_blank" >RIV/62156489:43210/10:00234796 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2010/mendelnet_2010_fulltext.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2010/mendelnet_2010_fulltext.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

THE USE OF MICROELUTION PLATE AS A PART OF HYPHENATED EXTRACTION METHOD FOLLOWED BY RRLC-MS-MS ANALYSIS FOR THE ISOLATION AND DETERMINATION OF PHENOLIC COMPOUNDS IN SEA ALGAE

Popis výsledku v původním jazyce

Development and aplication of uElution plate for the extraction of phenolic compounds from sea algae and their analysis using RRLC/MS/MS is described. The extraction and identification of phenolic compounds is presented from five different sea algae samples, two brown algae (Cystoseira abies + Cystoseira abies dried with nitrogen , Undaria pinnatifida) and two red algae (Sargassum muticum, Chondrus crispus) via solid phase extraction (SPE) using Oasis uElution plate. Selected groups of benzoic acid derivatives (p-hydroxybenzoic, protocatechuic, gallic, vanillic and syringic acid), hydroxybenzaldehydes (4-hydroxybenzaldehyde and 3,4- dihydroxybenzaldehyde) and cinnamic acid derivatives (o-coumaric, p-coumaric, caffeic, ferulic, sinapic and chlorogenic acid) were investigated. Recoveries in range 96--100% were obtained, with LOQs 0.01--2.1 pg/inj and LODs 0.03--7.1 pg/inj, i.e. in the range of low um. The uSPE enabled to avoid the evaporation step and preconcentrate the analytes directly

Název v anglickém jazyce

THE USE OF MICROELUTION PLATE AS A PART OF HYPHENATED EXTRACTION METHOD FOLLOWED BY RRLC-MS-MS ANALYSIS FOR THE ISOLATION AND DETERMINATION OF PHENOLIC COMPOUNDS IN SEA ALGAE

Popis výsledku anglicky

Development and aplication of uElution plate for the extraction of phenolic compounds from sea algae and their analysis using RRLC/MS/MS is described. The extraction and identification of phenolic compounds is presented from five different sea algae samples, two brown algae (Cystoseira abies + Cystoseira abies dried with nitrogen , Undaria pinnatifida) and two red algae (Sargassum muticum, Chondrus crispus) via solid phase extraction (SPE) using Oasis uElution plate. Selected groups of benzoic acid derivatives (p-hydroxybenzoic, protocatechuic, gallic, vanillic and syringic acid), hydroxybenzaldehydes (4-hydroxybenzaldehyde and 3,4- dihydroxybenzaldehyde) and cinnamic acid derivatives (o-coumaric, p-coumaric, caffeic, ferulic, sinapic and chlorogenic acid) were investigated. Recoveries in range 96--100% were obtained, with LOQs 0.01--2.1 pg/inj and LODs 0.03--7.1 pg/inj, i.e. in the range of low um. The uSPE enabled to avoid the evaporation step and preconcentrate the analytes directly

Druh

D - Stať ve sborníku

CEP obor

GC - Pěstování rostlin, osevní postupy

OECD FORD obor

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Projekt

<a href="/cs/project/GP525%2F08%2FP540" target="_blank" >GP525/08/P540: On-line spojení superkritické fluidní extrakce s extrakcí tuhou fází (SPE-SFE): optimalizace metody pro extrakci isoflavonů z biologické matrice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2010 - Proceedings of International Ph.D. Students Conference

ISBN

978-80-7375-453-2

ISSN

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e-ISSN

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Počet stran výsledku

14

Strana od-do

999-1012

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

24. 11. 2010

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

341786800131