The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F11%3A00166608" target="_blank" >RIV/62156489:43210/11:00166608 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR

Popis výsledku v původním jazyce

The selection of reference genes is essential for gene expression studies when using real-time quantitative PCR. Reference gene selection should be performed for each individual experiment because the gene expression level may change in different experimental conditions. In this study, the stability of mRNA expression was determined for seven genes: HPRT1, RPS18, NACA, TBP, TAF4B, RPL32 and OAZ1. The stability of these reference genes was investigated in the skeletal muscle tissue of pig foetuses, piglets and adult pigs using real-time quantitative PCR and SYBR green chemistry. The expression stability of used reference genes was calculated using geNorm application. Different gene expression profiles between the age categories of pigs were found. RPS18has been identified as the gene with the most stable expression in muscle tissue of all pig age categories. HPRT1 and RPL32 were found to have the highest stability in piglets and adult pigs, and in foetuses and adults pigs, respectively

Název v anglickém jazyce

The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR

Popis výsledku anglicky

The selection of reference genes is essential for gene expression studies when using real-time quantitative PCR. Reference gene selection should be performed for each individual experiment because the gene expression level may change in different experimental conditions. In this study, the stability of mRNA expression was determined for seven genes: HPRT1, RPS18, NACA, TBP, TAF4B, RPL32 and OAZ1. The stability of these reference genes was investigated in the skeletal muscle tissue of pig foetuses, piglets and adult pigs using real-time quantitative PCR and SYBR green chemistry. The expression stability of used reference genes was calculated using geNorm application. Different gene expression profiles between the age categories of pigs were found. RPS18has been identified as the gene with the most stable expression in muscle tissue of all pig age categories. HPRT1 and RPL32 were found to have the highest stability in piglets and adult pigs, and in foetuses and adults pigs, respectively

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA523%2F09%2F0844" target="_blank" >GA523/09/0844: K biologii růstu svalů u prasat: komparativní analýza genové exprese v kosterních svalech bílého ušlechtilého a divokého prasete</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Animal Science

ISSN

1212-1819

e-ISSN

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Svazek periodika

56

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

213-216

Kód UT WoS článku

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EID výsledku v databázi Scopus

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