Electrochemical behaviuor of (PRPC) and changed (PRPSC) prion protein

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical behaviuor of (PRPC) and changed (PRPSC) prion protein

Popis výsledku v původním jazyce

Prion diseases are fatal neurodegenerative and infectious disorders of humans and animals, characterized by structural transition of the host-encoded cellular prion protein (PrPC) into the aberrantly folded pathologic isoform PrPSc. Prion protein is a biomolecule naturally occurring in the animal cells. This protein is present in all mammal cells and occurs primarily in neural cells and immune system cells. The main aim of this study was to optimize electrochemical methods for the detection of natural (PrPC) and changed (PrPSC) prion protein. To carry out the main objective a complex study of the electrochemical behaviour of both proteins was required. For this purpose fundamental electrochemical techniques were used. Both of the prions were characterized using different techniques, their limits of detection were found at pM levels and possible ability to change the structure of alpha-helix of natural prion (PrPC) to beta-sheet of the infectious prion (PrPSc) were monitored.

Název v anglickém jazyce

Electrochemical behaviuor of (PRPC) and changed (PRPSC) prion protein

Popis výsledku anglicky

Prion diseases are fatal neurodegenerative and infectious disorders of humans and animals, characterized by structural transition of the host-encoded cellular prion protein (PrPC) into the aberrantly folded pathologic isoform PrPSc. Prion protein is a biomolecule naturally occurring in the animal cells. This protein is present in all mammal cells and occurs primarily in neural cells and immune system cells. The main aim of this study was to optimize electrochemical methods for the detection of natural (PrPC) and changed (PrPSC) prion protein. To carry out the main objective a complex study of the electrochemical behaviour of both proteins was required. For this purpose fundamental electrochemical techniques were used. Both of the prions were characterized using different techniques, their limits of detection were found at pM levels and possible ability to change the structure of alpha-helix of natural prion (PrPC) to beta-sheet of the infectious prion (PrPSc) were monitored.

Druh

D - Stať ve sborníku

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XI. pracovní setkání fyzikálních chemiků a elektrochemiků

ISBN

978-80-7375-514-0

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

279-281

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

MENDELU

Datum konání akce

1. 1. 2011

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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