Study of chalcon isomerase and anthocyanidin synthase DNA sequences in wheat (Triticum aestivum L.)

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Study of chalcon isomerase and anthocyanidin synthase DNA sequences in wheat (Triticum aestivum L.)

Popis výsledku v původním jazyce

Anthocyanins, a group of flavonoid substances that is responsible for colored caryopses of common wheat (Triticum aestivum L.). Pigments formed in anthocyanin biosynthetic pathway are deposited in different parts of the caryopsis. Purple anthocyanins areaccumulated in pericarp while blue anthocyanins are stored in aleurone layer of caryopsis. The spring wheat form of two genotypes with purple pericarp (Abyssinskaya arraseita and ANK-28B) and two genotypes with blue aleuron layer (Tschermaks Blaukörniger Sommerweizen and UC66049) were used in the experiments. Genotype with white caryopsis (Novosibirskaya 67) was used as a control. Total RNA was isolated from developing caryopses and transcribed into cDNA. Sequences for chalcon isomerase (98-100% similarity) and for anthocyanidin synthase genes were detected. The variability among genotypes in this study was due to insertion or deletion (indels). These candidate sequences were localized in the wheat genome and will be used for study of

Název v anglickém jazyce

Study of chalcon isomerase and anthocyanidin synthase DNA sequences in wheat (Triticum aestivum L.)

Popis výsledku anglicky

Anthocyanins, a group of flavonoid substances that is responsible for colored caryopses of common wheat (Triticum aestivum L.). Pigments formed in anthocyanin biosynthetic pathway are deposited in different parts of the caryopsis. Purple anthocyanins areaccumulated in pericarp while blue anthocyanins are stored in aleurone layer of caryopsis. The spring wheat form of two genotypes with purple pericarp (Abyssinskaya arraseita and ANK-28B) and two genotypes with blue aleuron layer (Tschermaks Blaukörniger Sommerweizen and UC66049) were used in the experiments. Genotype with white caryopsis (Novosibirskaya 67) was used as a control. Total RNA was isolated from developing caryopses and transcribed into cDNA. Sequences for chalcon isomerase (98-100% similarity) and for anthocyanidin synthase genes were detected. The variability among genotypes in this study was due to insertion or deletion (indels). These candidate sequences were localized in the wheat genome and will be used for study of

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2013 - Proceedings of International PhD Students Conference

ISBN

978-80-7375-908-7

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

674-677

Název nakladatele

Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic

Místo vydání

Faculty of Agronomy

Místo konání akce

Mendelova univerzita v Brně

Datum konání akce

20. 11. 2013

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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