Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00209872" target="_blank" >RIV/62156489:43210/13:00209872 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses
Popis výsledku v původním jazyce
Anthocyanins are responsible for the coloration in shades of blue, purple and red of various body parts of plants. Wheat caryopses with nonstandard coloration, specifically genotypes UC66049 and Tschermaks Blaukörniger Sommerweizen with blue aleurone, ANK28B and Abyssinskaya arraseita with purple pericarp were used in the experiment. Genotype Novosibirskaya 67 was used as a standard because it does not synthesize any pigments. Total RNA was isolated from caryopses by the phenol-chloroform method and transcribed into cDNA. In dihydroflavonol reductase (DFR) gene sequences obtained from direct sequencing of PCR product were detected several indels and single nucleotide polymorphisms. The similarity among all sequences of analyzed genotypes and sequence obtained from National Center for Biotechnology Information (NCBI) ranged between 94.59 to 100 %. The gene expression of DFR in samples varied during maturation. For the control genotype Novosibirskaya 67 there was highest DFR gene express
Název v anglickém jazyce
Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses
Popis výsledku anglicky
Anthocyanins are responsible for the coloration in shades of blue, purple and red of various body parts of plants. Wheat caryopses with nonstandard coloration, specifically genotypes UC66049 and Tschermaks Blaukörniger Sommerweizen with blue aleurone, ANK28B and Abyssinskaya arraseita with purple pericarp were used in the experiment. Genotype Novosibirskaya 67 was used as a standard because it does not synthesize any pigments. Total RNA was isolated from caryopses by the phenol-chloroform method and transcribed into cDNA. In dihydroflavonol reductase (DFR) gene sequences obtained from direct sequencing of PCR product were detected several indels and single nucleotide polymorphisms. The similarity among all sequences of analyzed genotypes and sequence obtained from National Center for Biotechnology Information (NCBI) ranged between 94.59 to 100 %. The gene expression of DFR in samples varied during maturation. For the control genotype Novosibirskaya 67 there was highest DFR gene express
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
MendelNet 2013 - Proceedings of International PhD Students Conference
ISBN
978-80-7375-908-7
ISSN
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e-ISSN
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Počet stran výsledku
5
Strana od-do
678-682
Název nakladatele
Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
Místo vydání
Faculty of Agronomy
Místo konání akce
Mendelova univerzita v Brně
Datum konání akce
20. 11. 2013
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
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