Femtogram electrochemical sensing of prion proteins using quantum dots

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00213512" target="_blank" >RIV/62156489:43210/13:00213512 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/13:00072405 RIV/00216305:26620/13:PU105964

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Femtogram electrochemical sensing of prion proteins using quantum dots

Popis výsledku v původním jazyce

The prion protein (PrP) is involved in neurodegeneration via its conversion from the normal cellular form, PrPC, to the infectious form, PrPSc, which is the causative agent of the transmissible spongiform encephalopathies (TSEs) including Creutzfeldt-Jakob disease (CJD). In spite of great effort in this field, diagnostics of prion protein caused diseases represents a sort of challenge. In this study, we aimed our attention on studying of prion protein interaction with CdTe quantum dots (QDs) by voltammetry as a new and extremely sensitive tool for sensing of these proteins. Primarily, we characterized fluorescent and electrochemical properties of QDs. Further, electrochemical study of their interactions was carried out to find the most suitable conditions for sensitive detection of prion proteins. Detection limit (3 S/N) was estimated as 1 fg in 5 ul. This makes labeling of proteins with QDs of great importance due to easy applicability and possibility to use in miniaturized devices, w

Název v anglickém jazyce

Femtogram electrochemical sensing of prion proteins using quantum dots

Popis výsledku anglicky

The prion protein (PrP) is involved in neurodegeneration via its conversion from the normal cellular form, PrPC, to the infectious form, PrPSc, which is the causative agent of the transmissible spongiform encephalopathies (TSEs) including Creutzfeldt-Jakob disease (CJD). In spite of great effort in this field, diagnostics of prion protein caused diseases represents a sort of challenge. In this study, we aimed our attention on studying of prion protein interaction with CdTe quantum dots (QDs) by voltammetry as a new and extremely sensitive tool for sensing of these proteins. Primarily, we characterized fluorescent and electrochemical properties of QDs. Further, electrochemical study of their interactions was carried out to find the most suitable conditions for sensitive detection of prion proteins. Detection limit (3 S/N) was estimated as 1 fg in 5 ul. This makes labeling of proteins with QDs of great importance due to easy applicability and possibility to use in miniaturized devices, w

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

10

Strana od-do

12466-12475

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—