The role of nitric oxide modulators in uptake of cadmium by chamomile plants (Matricaria chamomilla, asteraceae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A43908891" target="_blank" >RIV/62156489:43210/13:43908891 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The role of nitric oxide modulators in uptake of cadmium by chamomile plants (Matricaria chamomilla, asteraceae)

Popis výsledku v původním jazyce

The role of nitric oxide (NO) under Cd excess in chamomile (Matricaria chamomilla) was studied using known NO modulators (donor: sodium nitroprusside/SNP and scavenger: 2-phenyl-4,4,5,5-tetramethyl- imidazoline-1-oxyl-3-oxide/PTIO). Analyses showed thatthis modulator-enhanced Cd uptake depleted glutathione and partially ascorbic acid contents though it was expected that SNP should provide antioxidative protection through higher accumulation of antioxidants. Identification of phytochelatins using Orbitrap Elite system confirmed the occurrence of PC2 and PC3 and further LC-MS/MS quantification revealed depletion by SNP or PTIO application. In Cd treatment alone, glutathione and PCs increased concomitantly with NO generation, confirming their role in chamomile tolerance to metallic stress. These data provide the evidence that SNP/PTIO interacting with intact plants affect metal uptake and must therefore be used with caution.

Název v anglickém jazyce

The role of nitric oxide modulators in uptake of cadmium by chamomile plants (Matricaria chamomilla, asteraceae)

Popis výsledku anglicky

The role of nitric oxide (NO) under Cd excess in chamomile (Matricaria chamomilla) was studied using known NO modulators (donor: sodium nitroprusside/SNP and scavenger: 2-phenyl-4,4,5,5-tetramethyl- imidazoline-1-oxyl-3-oxide/PTIO). Analyses showed thatthis modulator-enhanced Cd uptake depleted glutathione and partially ascorbic acid contents though it was expected that SNP should provide antioxidative protection through higher accumulation of antioxidants. Identification of phytochelatins using Orbitrap Elite system confirmed the occurrence of PC2 and PC3 and further LC-MS/MS quantification revealed depletion by SNP or PTIO application. In Cd treatment alone, glutathione and PCs increased concomitantly with NO generation, confirming their role in chamomile tolerance to metallic stress. These data provide the evidence that SNP/PTIO interacting with intact plants affect metal uptake and must therefore be used with caution.

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2013: Proceedings of International PhD Students Conference

ISBN

978-80-7375-908-7

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

919-923

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

20. 11. 2013

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000366464800162