Unexpected Behavior of Some Nitric Oxide Modulators under Cadmium Excess in Plant Tissue

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A00218672" target="_blank" >RIV/62156489:43210/14:00218672 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16370/14:43872690

Výsledek na webu

<a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091685" target="_blank" >http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091685</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0091685" target="_blank" >10.1371/journal.pone.0091685</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Unexpected Behavior of Some Nitric Oxide Modulators under Cadmium Excess in Plant Tissue

Popis výsledku v původním jazyce

Various nitric oxide modulators (NO donors - SNP, GSNO, DEA NONOate and scavengers -- PTIO, cPTIO) were tested to highlight the role of NO under Cd excess in various ontogenetic stages of chamomile (Matricaria chamomilla). Surprisingly, compared to Cd alone, SNP and PTIO elevated Cd uptake (confirmed also by PhenGreen staining) but depleted glutathione (partially ascorbic acid) and phytochelatins PC2 and PC3 in both older plants (cultured hydroponically) and seedlings (cultured in deionised water). Despite these anomalous impacts, fluorescence staining of NO and ROS confirmed predictable assumptions and revealed reciprocal changes (decrease in NO but increase in ROS after PTIO addition and the opposite after SNP application). Subsequent tests using alternative modulators and seedlings confirmed changes to NO and ROS after application of GSNO and DEA NONOate as mentioned above for SNP while cPTIO altered only NO level (depletion). On the contrary to SNP and PTIO, GSNO, DEA NONOate and c

Název v anglickém jazyce

Unexpected Behavior of Some Nitric Oxide Modulators under Cadmium Excess in Plant Tissue

Popis výsledku anglicky

Various nitric oxide modulators (NO donors - SNP, GSNO, DEA NONOate and scavengers -- PTIO, cPTIO) were tested to highlight the role of NO under Cd excess in various ontogenetic stages of chamomile (Matricaria chamomilla). Surprisingly, compared to Cd alone, SNP and PTIO elevated Cd uptake (confirmed also by PhenGreen staining) but depleted glutathione (partially ascorbic acid) and phytochelatins PC2 and PC3 in both older plants (cultured hydroponically) and seedlings (cultured in deionised water). Despite these anomalous impacts, fluorescence staining of NO and ROS confirmed predictable assumptions and revealed reciprocal changes (decrease in NO but increase in ROS after PTIO addition and the opposite after SNP application). Subsequent tests using alternative modulators and seedlings confirmed changes to NO and ROS after application of GSNO and DEA NONOate as mentioned above for SNP while cPTIO altered only NO level (depletion). On the contrary to SNP and PTIO, GSNO, DEA NONOate and c

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.30.0017" target="_blank" >EE2.3.30.0017: Postdoktorandi v oborech biologických věd na MENDELU</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS One

ISSN

1932-6203

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

332851300120

EID výsledku v databázi Scopus

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