Granule-bound starch synthase and endosperm mealiness correlaton in wheat (Triticum aestivum L.) with nonstandard colored caryopses

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Granule-bound starch synthase and endosperm mealiness correlaton in wheat (Triticum aestivum L.) with nonstandard colored caryopses

Popis výsledku v původním jazyce

Granule-bound starch synthase (GBSS) is a key enzyme in starch biosynthesis pathway. It is responsible for production of amylose, therefore low amylose level has been associated with GBSS deficiency. This enzyme, also known as waxy protein, is encoded bythree homologous genes -- Wx-A1, Wx-B1 and Wx-D1 and their dysfunction of any origin causes decrease of amylose content. Starch content and, therefore amylose content, is closely linked with structure of the cereal endosperm. It is known, that mealy endosperm contains more starch than vitreous, which contains more proteins. To identify null alleles of waxy genes in 25 genotypes of wheat with nonstandard colored caryopses, PCR markers were used. To determine the endosperm structure of these genotypes, the non-destructive Light Transflectance Meter (LTm) was used. Null alleles for Wx-B1 locus were observed in 5 tested genotypes, loci Wx-A1 and Wx-D1 were amplified in all genotypes. Percentage of mealy caryopses per sample ranged from 0 t

Název v anglickém jazyce

Granule-bound starch synthase and endosperm mealiness correlaton in wheat (Triticum aestivum L.) with nonstandard colored caryopses

Popis výsledku anglicky

Granule-bound starch synthase (GBSS) is a key enzyme in starch biosynthesis pathway. It is responsible for production of amylose, therefore low amylose level has been associated with GBSS deficiency. This enzyme, also known as waxy protein, is encoded bythree homologous genes -- Wx-A1, Wx-B1 and Wx-D1 and their dysfunction of any origin causes decrease of amylose content. Starch content and, therefore amylose content, is closely linked with structure of the cereal endosperm. It is known, that mealy endosperm contains more starch than vitreous, which contains more proteins. To identify null alleles of waxy genes in 25 genotypes of wheat with nonstandard colored caryopses, PCR markers were used. To determine the endosperm structure of these genotypes, the non-destructive Light Transflectance Meter (LTm) was used. Null alleles for Wx-B1 locus were observed in 5 tested genotypes, loci Wx-A1 and Wx-D1 were amplified in all genotypes. Percentage of mealy caryopses per sample ranged from 0 t

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2014 - Proceedings of International PhD Students Conference

ISBN

978-80-7509-174-1

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

479-483

Název nakladatele

Mendel University in Brno

Místo vydání

Brno, Czech Republic

Místo konání akce

Brno, Czech Republic

Datum konání akce

19. 11. 2014

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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