Interaction study of arsenic (III and V) ions with metallothionein gene (MT2A) fragment
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906304" target="_blank" >RIV/62156489:43210/15:43906304 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216305:26620/15:PU112621
Výsledek na webu
<a href="http://www.sciencedirect.com/science/article/pii/S0141813014005972" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0141813014005972</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2014.09.001" target="_blank" >10.1016/j.ijbiomac.2014.09.001</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Interaction study of arsenic (III and V) ions with metallothionein gene (MT2A) fragment
Popis výsledku v původním jazyce
Arsenic compounds belong to the most controversial agents concerning human health. Arsenic (As) is considered as a top environmental element influencing human health due to its adverse effects including cancer, diabetes, cardiovascular disease, and reproductive or developmental problems. Despite the proven mutagenic, teratogenic and carcinogenic effects, the arsenic compounds are used for centuries to treat infectious diseases. In our work, we focused on studying of interactions of As(III) and/or As(V)with DNA. Interactions between arsenic ions and DNA were monitored by UV/vis spectrophotometry by measuring absorption and fluorescence spectra, atomic absorption spectrometry, electrochemical measurements (square wave voltammetry) and agarose gel electrophoresis. Using these methods, we observed a stable structure of DNA with As(III) within the concentration range 0.4-6.25 mu g mL(-1). Higher As(III) concentration caused degradation of DNA. However, similar effects were not observed for
Název v anglickém jazyce
Interaction study of arsenic (III and V) ions with metallothionein gene (MT2A) fragment
Popis výsledku anglicky
Arsenic compounds belong to the most controversial agents concerning human health. Arsenic (As) is considered as a top environmental element influencing human health due to its adverse effects including cancer, diabetes, cardiovascular disease, and reproductive or developmental problems. Despite the proven mutagenic, teratogenic and carcinogenic effects, the arsenic compounds are used for centuries to treat infectious diseases. In our work, we focused on studying of interactions of As(III) and/or As(V)with DNA. Interactions between arsenic ions and DNA were monitored by UV/vis spectrophotometry by measuring absorption and fluorescence spectra, atomic absorption spectrometry, electrochemical measurements (square wave voltammetry) and agarose gel electrophoresis. Using these methods, we observed a stable structure of DNA with As(III) within the concentration range 0.4-6.25 mu g mL(-1). Higher As(III) concentration caused degradation of DNA. However, similar effects were not observed for
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/EE2.3.20.0148" target="_blank" >EE2.3.20.0148: Mezinárodní spolupráce v oblasti "in vivo" zobrazovacích technik</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
International Journal of Biological Macromolecules
ISSN
0141-8130
e-ISSN
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Svazek periodika
72
Číslo periodika v rámci svazku
January
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
7
Strana od-do
599-605
Kód UT WoS článku
000347577900078
EID výsledku v databázi Scopus
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