A comparison of Biuret, Lowry and Bradford methods for measuring the egg's proteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906671" target="_blank" >RIV/62156489:43210/15:43906671 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A comparison of Biuret, Lowry and Bradford methods for measuring the egg's proteins

Popis výsledku v původním jazyce

Quantitation of the total protein content in a sample is a critical step in protein analysis. MolecularUV-Vis absorption spectroscopy is very efficient in quantitative analysis such as protein quantitation and has extensive applications in chemical and biochemical laboratories, medicine and food industry. Traditional spectroscopic methods are cheap, easy-working and the most common way to quantitate protein concentrations. This study compares Biuret, Lowry and Bradford methods for measuring hen albumenand egg yolk as protein samples. These methods are commonly used for determination proteins. The Biuret test uses as a reagent: Biuret reagent. For Lowry assay are used four reagents: reagent A, reagent B, reagent C and reagent D. For last method, Bradford, is used as a reagent Coomassie Brilliant Blue G-250. The absorbance was measured at a wavelength of 750nm for Lowry, 540 nm for Biuret and 595 nm for Bradford assay. The lowest content of proteins was analysed in albumen (0.706 mg.ml-

Název v anglickém jazyce

A comparison of Biuret, Lowry and Bradford methods for measuring the egg's proteins

Popis výsledku anglicky

Quantitation of the total protein content in a sample is a critical step in protein analysis. MolecularUV-Vis absorption spectroscopy is very efficient in quantitative analysis such as protein quantitation and has extensive applications in chemical and biochemical laboratories, medicine and food industry. Traditional spectroscopic methods are cheap, easy-working and the most common way to quantitate protein concentrations. This study compares Biuret, Lowry and Bradford methods for measuring hen albumenand egg yolk as protein samples. These methods are commonly used for determination proteins. The Biuret test uses as a reagent: Biuret reagent. For Lowry assay are used four reagents: reagent A, reagent B, reagent C and reagent D. For last method, Bradford, is used as a reagent Coomassie Brilliant Blue G-250. The absorbance was measured at a wavelength of 750nm for Lowry, 540 nm for Biuret and 595 nm for Bradford assay. The lowest content of proteins was analysed in albumen (0.706 mg.ml-

Druh

D - Stať ve sborníku

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2015: Proceedings of International PhD Students Conference

ISBN

978-80-7509-363-9

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

394-398

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000366466100072