Fluorescence-tagged metallothionein with CdTe quantum dots analyzed by the chip-CE technique

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906766" target="_blank" >RIV/62156489:43210/15:43906766 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/content/pdf/10.1007%2Fs11051-015-3226-8.pdf" target="_blank" >http://link.springer.com/content/pdf/10.1007%2Fs11051-015-3226-8.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11051-015-3226-8" target="_blank" >10.1007/s11051-015-3226-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fluorescence-tagged metallothionein with CdTe quantum dots analyzed by the chip-CE technique

Popis výsledku v původním jazyce

Quantum dots (QDs) are fluorescence nanoparticles (NPs) with unique optic properties which allow their use as probes in chemical, biological, immunological, and molecular imaging. QDs linked with target ligands such as peptides or small molecules can beused as tumor biomarkers. These particles are a promising tool for selective, fast, and sensitive tagging and imaging in medicine. In this study, an attempt was made to use QDs as a marker for human metallothionein (MT) isoforms 1 and 2. Four kinds of CdTe QDs of different sizes bioconjugated with MT were analyzed using the chip-CE technique. Based on the results, it can be concluded that MT is willing to interact with QDs, and the chip-CE technique enables the observation of their complexes. It was also observed that changes ranging roughly 6-7 kDa, a value corresponding to the MT monomer, depend on the hydrodynamic diameters of QDs; also, the MT sample without cadmium interacted stronger with QDs than MT saturated with cadmium. Result

Název v anglickém jazyce

Fluorescence-tagged metallothionein with CdTe quantum dots analyzed by the chip-CE technique

Popis výsledku anglicky

Quantum dots (QDs) are fluorescence nanoparticles (NPs) with unique optic properties which allow their use as probes in chemical, biological, immunological, and molecular imaging. QDs linked with target ligands such as peptides or small molecules can beused as tumor biomarkers. These particles are a promising tool for selective, fast, and sensitive tagging and imaging in medicine. In this study, an attempt was made to use QDs as a marker for human metallothionein (MT) isoforms 1 and 2. Four kinds of CdTe QDs of different sizes bioconjugated with MT were analyzed using the chip-CE technique. Based on the results, it can be concluded that MT is willing to interact with QDs, and the chip-CE technique enables the observation of their complexes. It was also observed that changes ranging roughly 6-7 kDa, a value corresponding to the MT monomer, depend on the hydrodynamic diameters of QDs; also, the MT sample without cadmium interacted stronger with QDs than MT saturated with cadmium. Result

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Nanoparticle Research

ISSN

1388-0764

e-ISSN

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Svazek periodika

17

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

000363982000001

EID výsledku v databázi Scopus

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