Effects of fish oil diet on M1 and M2 monocyte derived macrophages polarization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906869" target="_blank" >RIV/62156489:43210/15:43906869 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/15:#0001237

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of fish oil diet on M1 and M2 monocyte derived macrophages polarization

Popis výsledku v původním jazyce

The aim of this study was demonstrated effect of fish oil diet on M1 and M2 polarization of macrophages. Six piglets were fed with standard diet supplemented with 2.5% fish oil containing eicosapentaenoic acid (EPA) and six piglets were fed with addition2.5% palm oil as control group. We obtained mononuclear fraction of white blood cells from peripheral blood and we subsequently obtained CD14+ monocytes by magnetic separation. After 7 days of cultivation we obtained monocyte-derived macrophages (MDMF).It was measured genes expressions of pro-inflammatory soluble factors (IL-1?, TNF-? and MMP12) and anti-inflammatory (IL-10 and TIMP1) for detection of M1 or M2 polarization of MDMF. In the diet with fish oil, it showed a statistically significant increase in gene expression of MMP12 (P<0.01). It was measured genes expressions after stimulation of lipopolysaccharide (LPS). In case of both diet (fish oil and palm oil) IL-1 gene expression was increased in contrast to HPRT-1 (housekeeping

Název v anglickém jazyce

Effects of fish oil diet on M1 and M2 monocyte derived macrophages polarization

Popis výsledku anglicky

The aim of this study was demonstrated effect of fish oil diet on M1 and M2 polarization of macrophages. Six piglets were fed with standard diet supplemented with 2.5% fish oil containing eicosapentaenoic acid (EPA) and six piglets were fed with addition2.5% palm oil as control group. We obtained mononuclear fraction of white blood cells from peripheral blood and we subsequently obtained CD14+ monocytes by magnetic separation. After 7 days of cultivation we obtained monocyte-derived macrophages (MDMF).It was measured genes expressions of pro-inflammatory soluble factors (IL-1?, TNF-? and MMP12) and anti-inflammatory (IL-10 and TIMP1) for detection of M1 or M2 polarization of MDMF. In the diet with fish oil, it showed a statistically significant increase in gene expression of MMP12 (P<0.01). It was measured genes expressions after stimulation of lipopolysaccharide (LPS). In case of both diet (fish oil and palm oil) IL-1 gene expression was increased in contrast to HPRT-1 (housekeeping

Druh

D - Stať ve sborníku

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/LO1218" target="_blank" >LO1218: Zdravé zvíře jako zdroj zdravé potraviny</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2015: Proceedings of International PhD Students Conference

ISBN

978-80-7509-363-9

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

384-388

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000366466100070