Rapid identification of bacteria by biobarcode assay

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906879" target="_blank" >RIV/62156489:43210/15:43906879 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Rapid identification of bacteria by biobarcode assay

Popis výsledku v původním jazyce

Presence of bacteria with antibiotic resistance is becoming a very large problem throughout the world. Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen resistant to ?-lactam antibiotics with biofilm-formation ability. Because ofan increasing resistance of bacterial species to ATBs, it is necessary to develop new methods for rapid identification of bacteria. Biobarcode assay provides a rapid detection of the antigen presence in the sample. Detection is based on the antibody-antigen interaction. The antibodies were bound to magnetic and non-magnetic particles. Next, immunoglobulin G (IgG) was bounded to magnetic particles. The non-magnetic particles were bound with anti-plasminogen antibody that is a specific antigen for MRSA as well as 20 bp oligonucleotides for detection. The first step involved determination of binding capacity of antibodies for different bacteria by ELISA. The IgG were able to bind 4.6 .104 +- 8% CFU . ml-1 of MRSA, Escherichia coli and Pro

Název v anglickém jazyce

Rapid identification of bacteria by biobarcode assay

Popis výsledku anglicky

Presence of bacteria with antibiotic resistance is becoming a very large problem throughout the world. Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen resistant to ?-lactam antibiotics with biofilm-formation ability. Because ofan increasing resistance of bacterial species to ATBs, it is necessary to develop new methods for rapid identification of bacteria. Biobarcode assay provides a rapid detection of the antigen presence in the sample. Detection is based on the antibody-antigen interaction. The antibodies were bound to magnetic and non-magnetic particles. Next, immunoglobulin G (IgG) was bounded to magnetic particles. The non-magnetic particles were bound with anti-plasminogen antibody that is a specific antigen for MRSA as well as 20 bp oligonucleotides for detection. The first step involved determination of binding capacity of antibodies for different bacteria by ELISA. The IgG were able to bind 4.6 .104 +- 8% CFU . ml-1 of MRSA, Escherichia coli and Pro

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2015: Proceedings of International PhD Students Conference

ISBN

978-80-7509-363-9

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

448-452

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000366466100084