Particle-based immunochemical separation of methicillin resistant Staphylococcus aureus with indirect electrochemical detection of labeling oligonucleotides

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43910481" target="_blank" >RIV/62156489:43210/16:43910481 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/16:PU119672

Výsledek na webu

<a href="http://dx.doi.org/10.1039/c6ay01296e" target="_blank" >http://dx.doi.org/10.1039/c6ay01296e</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c6ay01296e" target="_blank" >10.1039/c6ay01296e</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Particle-based immunochemical separation of methicillin resistant Staphylococcus aureus with indirect electrochemical detection of labeling oligonucleotides

Popis výsledku v původním jazyce

Early detection of antibiotic-resistant bacteria causing inflammation in patients is a key for an appropriate and timely treatment. Classical microbiological cultivation methods are time-consuming and therefore it is necessary to discover a quick and effective method. The method used in this study is based on a sandwich immunoreaction of bacteria with IgG immobilized on streptavidin-coated polystyrene superparamagnetic particles and MRSA-specific antibody immobilized on gold non-magnetic nanoparticles labeled with oligonucleotides, which were detected by square wave voltammetry in combination with the adsorptive transfer technique. The assay is suitable for selective detection of methicillin-resistant Staphylococcus aureus in samples even at a concentration of 2 x 10(4) CFU mL(-1). The method can be flexibly modified for any other bacteria, depending on the antibodies used.

Název v anglickém jazyce

Particle-based immunochemical separation of methicillin resistant Staphylococcus aureus with indirect electrochemical detection of labeling oligonucleotides

Popis výsledku anglicky

Early detection of antibiotic-resistant bacteria causing inflammation in patients is a key for an appropriate and timely treatment. Classical microbiological cultivation methods are time-consuming and therefore it is necessary to discover a quick and effective method. The method used in this study is based on a sandwich immunoreaction of bacteria with IgG immobilized on streptavidin-coated polystyrene superparamagnetic particles and MRSA-specific antibody immobilized on gold non-magnetic nanoparticles labeled with oligonucleotides, which were detected by square wave voltammetry in combination with the adsorptive transfer technique. The assay is suitable for selective detection of methicillin-resistant Staphylococcus aureus in samples even at a concentration of 2 x 10(4) CFU mL(-1). The method can be flexibly modified for any other bacteria, depending on the antibodies used.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Methods

ISSN

1759-9660

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

25

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

5123-5128

Kód UT WoS článku

000378941500020

EID výsledku v databázi Scopus

2-s2.0-84976417386