Preparation and testing of a specific bionanosensor for detection of Staphylococcus aureus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F21%3A00354928" target="_blank" >RIV/68407700:21460/21:00354928 - isvavai.cz</a>

Výsledek na webu

<a href="http://imbm.fbmi.cvut.cz/soubory/Sborniky/IMBM%202021" target="_blank" >http://imbm.fbmi.cvut.cz/soubory/Sborniky/IMBM%202021</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preparation and testing of a specific bionanosensor for detection of Staphylococcus aureus

Popis výsledku v původním jazyce

Today, the detection of bacteria is mainly performed using metabolic tests, PCR and ELISA. However, these methods are time consuming, so new methods of so-called online detection are being sought. Biosensors are very fast, sensitive and accurate devices that enable the mentioned online detection. The aim of this work was to design and create a prototype of the biosensor that would be used to detect Staphylococcus aureus from the air. This sensor was made of PAN nanofibers (polyacrylonitrile) as a carrier and an antibody as a biosensitive layer. Furthermore, a system for an air filtration was created, by means of which detection was realized. The formed system was tested using PVA nanofibers (polyvinyl alcohol) of 1 g/m2 and fluorescence spectroscopy. Subsequent detection of the bacteria was performed using the already mentioned immobilized PAN nanofibers and pure PAN nanofibers as a control. The assays were evaluated by measuring the optical density (OD) of a bacterial suspension formed from bacteria captured on the sensor. Finally, the measurement of electrical properties (resistance, capacity) of the model case (PAN nanofibers and cigarette smoke particles) was also described.

Název v anglickém jazyce

Preparation and testing of a specific bionanosensor for detection of Staphylococcus aureus

Popis výsledku anglicky

Today, the detection of bacteria is mainly performed using metabolic tests, PCR and ELISA. However, these methods are time consuming, so new methods of so-called online detection are being sought. Biosensors are very fast, sensitive and accurate devices that enable the mentioned online detection. The aim of this work was to design and create a prototype of the biosensor that would be used to detect Staphylococcus aureus from the air. This sensor was made of PAN nanofibers (polyacrylonitrile) as a carrier and an antibody as a biosensitive layer. Furthermore, a system for an air filtration was created, by means of which detection was realized. The formed system was tested using PVA nanofibers (polyvinyl alcohol) of 1 g/m2 and fluorescence spectroscopy. Subsequent detection of the bacteria was performed using the already mentioned immobilized PAN nanofibers and pure PAN nanofibers as a control. The assays were evaluated by measuring the optical density (OD) of a bacterial suspension formed from bacteria captured on the sensor. Finally, the measurement of electrical properties (resistance, capacity) of the model case (PAN nanofibers and cigarette smoke particles) was also described.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů