The use of functionalized nanofiber membrane as a system for Escherichia coli detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F22%3A00361033" target="_blank" >RIV/68407700:21460/22:00361033 - isvavai.cz</a>

Výsledek na webu

<a href="http://imbm.fbmi.cvut.cz/soubory/Sborniky/IMBM%202022" target="_blank" >http://imbm.fbmi.cvut.cz/soubory/Sborniky/IMBM%202022</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The use of functionalized nanofiber membrane as a system for Escherichia coli detection

Popis výsledku v původním jazyce

Due to its widespread occurrence, Escherichia coli is one of the best-studied microorganisms. Although we encounter this bacterium primarily as a symbiotic organism, it can cause several problems. Among the diseases caused by E. coli are mainly urinary tract infections, septicemia, pneumonia, peritonitis, gastroenteritis, and neonatal meningitis. In addition to these pathological conditions, E. coli is also responsible for food-borne diseases. As with other pathogens, rapid and easy detection is essential for proper and effective treatment. This work aimed to design a new and portable method suitable for fast detection of Escherichia coli. Using antibody-functionalized PAN nanofibers, bacteria were successfully captured and detected from the air, and the experiments were evaluated by measuring the optical density of created bacterial suspensions. The experiment results show that the functionalization of the nanofiber membrane increased efficiency by up to 30 % compared to unmodified nanofibers. Thus, the modified nanofibers bring an attractive solution for the biological sensing of pathogenic microorganisms.

Název v anglickém jazyce

The use of functionalized nanofiber membrane as a system for Escherichia coli detection

Popis výsledku anglicky

Due to its widespread occurrence, Escherichia coli is one of the best-studied microorganisms. Although we encounter this bacterium primarily as a symbiotic organism, it can cause several problems. Among the diseases caused by E. coli are mainly urinary tract infections, septicemia, pneumonia, peritonitis, gastroenteritis, and neonatal meningitis. In addition to these pathological conditions, E. coli is also responsible for food-borne diseases. As with other pathogens, rapid and easy detection is essential for proper and effective treatment. This work aimed to design a new and portable method suitable for fast detection of Escherichia coli. Using antibody-functionalized PAN nanofibers, bacteria were successfully captured and detected from the air, and the experiments were evaluated by measuring the optical density of created bacterial suspensions. The experiment results show that the functionalization of the nanofiber membrane increased efficiency by up to 30 % compared to unmodified nanofibers. Thus, the modified nanofibers bring an attractive solution for the biological sensing of pathogenic microorganisms.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů