Polymorphisms in plasma membrane calcium-transporting ATPase 1 (ATP2b1) gene in hens

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906881" target="_blank" >RIV/62156489:43210/15:43906881 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Polymorphisms in plasma membrane calcium-transporting ATPase 1 (ATP2b1) gene in hens

Popis výsledku v původním jazyce

Bone fragility in caged laying hens is a severe welfare problem. This fragility has been attributed to osteoporosis, which etiology is multifactorial in birds, as well as in humans, with genetic, environmental, and nutritional components. Plasma membranecalcium-transporting ATPase 1 gene (ATP2B1) is in hens located on chromosome 1, region 43 273 706 - 43 305 815 bp. This gene has 21 exons, three of them were genotyped. In this study we genotyped 110 hens of ISA BROWN hybrids. Genotypes of ATP2B1 gene were determined using PCR-RFLP in exons 10 and 12. Genotypes of two SNPs in exon 8 were determined using sequencing. In our group of animals, only allele without deletion in exon 10 and only allele A in exon 12 was found. In exon 8 subsequent genotypes were detected: in C61T locus CC and TT; in C80T locus CC, CT and TT.

Název v anglickém jazyce

Polymorphisms in plasma membrane calcium-transporting ATPase 1 (ATP2b1) gene in hens

Popis výsledku anglicky

Bone fragility in caged laying hens is a severe welfare problem. This fragility has been attributed to osteoporosis, which etiology is multifactorial in birds, as well as in humans, with genetic, environmental, and nutritional components. Plasma membranecalcium-transporting ATPase 1 gene (ATP2B1) is in hens located on chromosome 1, region 43 273 706 - 43 305 815 bp. This gene has 21 exons, three of them were genotyped. In this study we genotyped 110 hens of ISA BROWN hybrids. Genotypes of ATP2B1 gene were determined using PCR-RFLP in exons 10 and 12. Genotypes of two SNPs in exon 8 were determined using sequencing. In our group of animals, only allele without deletion in exon 10 and only allele A in exon 12 was found. In exon 8 subsequent genotypes were detected: in C61T locus CC and TT; in C80T locus CC, CT and TT.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2015: Proceedings of International PhD Students Conference

ISBN

978-80-7509-363-9

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

458-461

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000366466100086