The effect of probiotics on the viability of the porcine and human monocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906909" target="_blank" >RIV/62156489:43210/15:43906909 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2015/articles/64_vejrychova_1178.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2015/articles/64_vejrychova_1178.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of probiotics on the viability of the porcine and human monocytes

Popis výsledku v původním jazyce

The aim of this study was evaluate the effect of probiotics on the viability of the cells of immune system. For experiment we have chosen the monocytes, which were isolated from porcine and human blood. The population of monocytes was cultivated in vitroconditions with probiotics strains such as Bifidobacterium bifidum, Lactobacillus rhamnosus and Enterococcus faecium. The monocytes were incubated without (control sample) or with probiotics for 2 and 4 hours. The percentage of apoptosis and necrosis ofmonocytes was analysed by flow cytometry. The results have shown statistically significant differences in proportion of porcine apoptotic monocytes cultivated with Bifidobacterium bifidum. Enterococcus faecium showed statistically significant effect onnecrosis of porcine monocytes. The statistically significant differences in proportion of human apoptotic monocytes were observed in cultivation with Lactobacillus rhamnosus and Enterococcus faecium and the highest percentage of necrotic

Název v anglickém jazyce

The effect of probiotics on the viability of the porcine and human monocytes

Popis výsledku anglicky

The aim of this study was evaluate the effect of probiotics on the viability of the cells of immune system. For experiment we have chosen the monocytes, which were isolated from porcine and human blood. The population of monocytes was cultivated in vitroconditions with probiotics strains such as Bifidobacterium bifidum, Lactobacillus rhamnosus and Enterococcus faecium. The monocytes were incubated without (control sample) or with probiotics for 2 and 4 hours. The percentage of apoptosis and necrosis ofmonocytes was analysed by flow cytometry. The results have shown statistically significant differences in proportion of porcine apoptotic monocytes cultivated with Bifidobacterium bifidum. Enterococcus faecium showed statistically significant effect onnecrosis of porcine monocytes. The statistically significant differences in proportion of human apoptotic monocytes were observed in cultivation with Lactobacillus rhamnosus and Enterococcus faecium and the highest percentage of necrotic

Druh

D - Stať ve sborníku

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2015: Proceedings of International PhD Students Conference

ISBN

978-80-7509-363-9

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

494-499

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000366466100094