Evaluation of apoptosis and necrosis of peritoneal macrophages in rats after injection of zinc chelates into abdominal cavity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906916" target="_blank" >RIV/62156489:43210/15:43906916 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2015/articles/67_vavrova_1182.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2015/articles/67_vavrova_1182.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of apoptosis and necrosis of peritoneal macrophages in rats after injection of zinc chelates into abdominal cavity

Popis výsledku v původním jazyce

Aim of this study was to evaluate the influence of zinc chelates injected into abdominal cavity on viability of peritoneal macrophages. Three organic acids (EDTA, DTPA, NTA) were used as zinc carriers. 24 female rats were employed in this study. The ratswere divided into 5 groups: 6 received intraperitoneal injection of 2 mL 40 mM Zn-EDTA (group E), 6 received intraperitoneal injection of 2 mL 40 mM Zn-DTPA (group D), 6 rats received intraperitoneal injection of 40 mM 2 mL Zn-NTA (group N), 3 rats received 2 mL of normal saline (group K4-6) and 3 rats (group K1-3 for control) were intact. On the day after injection all rats were sacrificed and peritoneal lavages were performed and cell viability analysis was done. The macrophages were divided in two morphologically different groups - group of smaller monocytes-like macrophages (ML) with kidney-shaped nuclei and pseudopodia on their surface, and a group of macrophages with spherical nuclei and many vacuoles in the cytoplasm (vacuolized

Název v anglickém jazyce

Evaluation of apoptosis and necrosis of peritoneal macrophages in rats after injection of zinc chelates into abdominal cavity

Popis výsledku anglicky

Aim of this study was to evaluate the influence of zinc chelates injected into abdominal cavity on viability of peritoneal macrophages. Three organic acids (EDTA, DTPA, NTA) were used as zinc carriers. 24 female rats were employed in this study. The ratswere divided into 5 groups: 6 received intraperitoneal injection of 2 mL 40 mM Zn-EDTA (group E), 6 received intraperitoneal injection of 2 mL 40 mM Zn-DTPA (group D), 6 rats received intraperitoneal injection of 40 mM 2 mL Zn-NTA (group N), 3 rats received 2 mL of normal saline (group K4-6) and 3 rats (group K1-3 for control) were intact. On the day after injection all rats were sacrificed and peritoneal lavages were performed and cell viability analysis was done. The macrophages were divided in two morphologically different groups - group of smaller monocytes-like macrophages (ML) with kidney-shaped nuclei and pseudopodia on their surface, and a group of macrophages with spherical nuclei and many vacuoles in the cytoplasm (vacuolized

Druh

D - Stať ve sborníku

CEP obor

GH - Výživa hospodářských zvířat

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2015: Proceedings of International PhD Students Conference

ISBN

978-80-7509-363-9

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

600-603

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000366466100113