Seed proteome analysis and proteome dynamics during seed germination

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906943" target="_blank" >RIV/62156489:43210/15:43906943 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Seed proteome analysis and proteome dynamics during seed germination

Popis výsledku v původním jazyce

Despite the huge progress that has been made in the last decade, the molecular mechanisms regulating seed germination and early seed development are far from being resolved. Induction of metabolic genes involved in germination starts around 12 hours after imbibition. Thus, most of the early events are mediated by molecules stored in the seed during maturation and are not accessible to transcriptomic analyses. Proteome analysis has been extensively employed in the past but the coverage of observed seed proteome is relatively low even in present-day high-impact studies. Here, we analysed proteome of two model species, Arabidopsis thaliana and barley (Hordeum vulgare). We employed several complementary approaches to increase the proteome coverage and build a library suitable for targeted protein quantitation. The combination of fractionations and an alternative MS/MS data processing significantly improved our detection limits. Our results indicate that the seed proteome coverage is limite

Název v anglickém jazyce

Seed proteome analysis and proteome dynamics during seed germination

Popis výsledku anglicky

Despite the huge progress that has been made in the last decade, the molecular mechanisms regulating seed germination and early seed development are far from being resolved. Induction of metabolic genes involved in germination starts around 12 hours after imbibition. Thus, most of the early events are mediated by molecules stored in the seed during maturation and are not accessible to transcriptomic analyses. Proteome analysis has been extensively employed in the past but the coverage of observed seed proteome is relatively low even in present-day high-impact studies. Here, we analysed proteome of two model species, Arabidopsis thaliana and barley (Hordeum vulgare). We employed several complementary approaches to increase the proteome coverage and build a library suitable for targeted protein quantitation. The combination of fractionations and an alternative MS/MS data processing significantly improved our detection limits. Our results indicate that the seed proteome coverage is limite

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2015: Proceedings of International PhD Students Conference

ISBN

978-80-7509-363-9

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

412-415

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000366466100076