An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43907666" target="_blank" >RIV/62156489:43210/15:43907666 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/15:PU118492

Výsledek na webu

<a href="http://link.springer.com/article/10.1007/s00604-015-1502-z/fulltext.html" target="_blank" >http://link.springer.com/article/10.1007/s00604-015-1502-z/fulltext.html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00604-015-1502-z" target="_blank" >10.1007/s00604-015-1502-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA

Popis výsledku v původním jazyce

A DNA-based biosensor is presented that can be applied to the detection of DNA damage caused by UV-C radiation (254 nm) in the presence of CdTe quantum dots (QDs). The sensor is composed of a glassy carbon electrode whose surface was modified with a layer of dsDNA and another layer of CdTe QDs. The response of this sensor is based on (a) the intrinsic anodic signal of the guanine moiety in the DNA that is measured by square-wave voltammetry, and (b) the cyclic voltammetric response of the redox indicator system hexacyanoferrate(III/II). Depending on the size of the QDs, they exert a significant effect on the rate of the degradation of dsDNA by UV-C light, and even by visible light. Time-dependent structural changes of DNA include opening of the doublehelix (as indicated by an increase in the redox response of the guanine moiety due to easy electron exchange with the electrode when compared to the original helix state and by an increase in the voltammetric peak current of the hexacyano

Název v anglickém jazyce

An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA

Popis výsledku anglicky

A DNA-based biosensor is presented that can be applied to the detection of DNA damage caused by UV-C radiation (254 nm) in the presence of CdTe quantum dots (QDs). The sensor is composed of a glassy carbon electrode whose surface was modified with a layer of dsDNA and another layer of CdTe QDs. The response of this sensor is based on (a) the intrinsic anodic signal of the guanine moiety in the DNA that is measured by square-wave voltammetry, and (b) the cyclic voltammetric response of the redox indicator system hexacyanoferrate(III/II). Depending on the size of the QDs, they exert a significant effect on the rate of the degradation of dsDNA by UV-C light, and even by visible light. Time-dependent structural changes of DNA include opening of the doublehelix (as indicated by an increase in the redox response of the guanine moiety due to easy electron exchange with the electrode when compared to the original helix state and by an increase in the voltammetric peak current of the hexacyano

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchimica Acta

ISSN

0026-3672

e-ISSN

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Svazek periodika

182

Číslo periodika v rámci svazku

9-10

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

8

Strana od-do

1715-1722

Kód UT WoS článku

000356449700017

EID výsledku v databázi Scopus

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