Prion protein and its interactions with metal ions (Cu2 , Zn2 , and Cd2 ) and metallothionein 3

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43907826" target="_blank" >RIV/62156489:43210/15:43907826 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/15:PU136027

Výsledek na webu

<a href="http://dx.doi.org/10.5599/admet.3.3.191" target="_blank" >http://dx.doi.org/10.5599/admet.3.3.191</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5599/admet.3.3.191" target="_blank" >10.5599/admet.3.3.191</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Prion protein and its interactions with metal ions (Cu2 , Zn2 , and Cd2 ) and metallothionein 3

Popis výsledku v původním jazyce

The effects of heavy metals (Zn2+, Cu2+, and/or Cd2+) on Escherichia coli expressing either prion (hPrPC ) or metallothionein 3 (MT-3) brain proteins capable of binding these metals were investigated. The expression of hPrPC or MT-3 in E.coli was confirmed using western-blot and dot-blot methods. After analyzing growth curves, we found that bacteria expressing prion protein better tolerated the presence of Zn2+ in comparison with wild-type bacteria and bacteria expressing MT-3. The addition of Cd2+ and Cu2+ was well tolerated by bacteria expressing MT-3, whereas the bacteria expressing prion protein displayed slower growth when compared to the wild-type. We subsequently determined total content of the MT in bacteria using differential pulsed voltammetry (DPV), and depending on the treatment of the individual metals. MT expression in MT3 transformed cells as well as in control E.coli cells increased at the lowest metal concentration (25 μM), followed by a decrease at higher metal concentrations (50, 75, and 150 μM). The highest increase by Cd2+ were observed. MT expression pattern in hPrPC transformed cells was different. After application of Cu2+ an increase in MT expression continued also at higher metal concentrations; and after application of Cd2+ and Zn2+ no decrease in MT expression at higher metal concentrations was observed

Název v anglickém jazyce

Prion protein and its interactions with metal ions (Cu2 , Zn2 , and Cd2 ) and metallothionein 3

Popis výsledku anglicky

The effects of heavy metals (Zn2+, Cu2+, and/or Cd2+) on Escherichia coli expressing either prion (hPrPC ) or metallothionein 3 (MT-3) brain proteins capable of binding these metals were investigated. The expression of hPrPC or MT-3 in E.coli was confirmed using western-blot and dot-blot methods. After analyzing growth curves, we found that bacteria expressing prion protein better tolerated the presence of Zn2+ in comparison with wild-type bacteria and bacteria expressing MT-3. The addition of Cd2+ and Cu2+ was well tolerated by bacteria expressing MT-3, whereas the bacteria expressing prion protein displayed slower growth when compared to the wild-type. We subsequently determined total content of the MT in bacteria using differential pulsed voltammetry (DPV), and depending on the treatment of the individual metals. MT expression in MT3 transformed cells as well as in control E.coli cells increased at the lowest metal concentration (25 μM), followed by a decrease at higher metal concentrations (50, 75, and 150 μM). The highest increase by Cd2+ were observed. MT expression pattern in hPrPC transformed cells was different. After application of Cu2+ an increase in MT expression continued also at higher metal concentrations; and after application of Cd2+ and Zn2+ no decrease in MT expression at higher metal concentrations was observed

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ADMET &amp; DMPK

ISSN

1848-7718

e-ISSN

—

Svazek periodika

3

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

HR - Chorvatská republika

Počet stran výsledku

9

Strana od-do

287-295

Kód UT WoS článku

000447335900013

EID výsledku v databázi Scopus

2-s2.0-85047052672