Influence of a storage protocol on sarcosine levels in the human urinary specimens

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43909563" target="_blank" >RIV/62156489:43210/15:43909563 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Influence of a storage protocol on sarcosine levels in the human urinary specimens

Popis výsledku v původním jazyce

Urinary metabolomic profiles have recently drawn a lot of attention owing to a debate regarding their possible role as potential clinical markers for prostate cancer. As was shown, amino acid metabolism in cancer patients differs from that in healthy people, and it can be thus utilized in early diagnostics. In this study, we monitored the behavior of potential noninvasive biomarker for prostate carcinoma, sarcosine, involved in the folate metabolism and DNA methylation processes, linked to the progression of prostate carcinoma. To obtain the maximum amount of information, the biochemical parameters (total protein, creatinine, ions, conductivity) were determined using spectrophotometry and electrochemistry. All results were subjected to statistical processing for revealing different correlations between the studied parameters. These metabolites were observed in the urine obtained from healthy subjects and influence of storage conditions (freezing and thawing) on the concentration of addition of sarcosine was monitored.

Název v anglickém jazyce

Influence of a storage protocol on sarcosine levels in the human urinary specimens

Popis výsledku anglicky

Urinary metabolomic profiles have recently drawn a lot of attention owing to a debate regarding their possible role as potential clinical markers for prostate cancer. As was shown, amino acid metabolism in cancer patients differs from that in healthy people, and it can be thus utilized in early diagnostics. In this study, we monitored the behavior of potential noninvasive biomarker for prostate carcinoma, sarcosine, involved in the folate metabolism and DNA methylation processes, linked to the progression of prostate carcinoma. To obtain the maximum amount of information, the biochemical parameters (total protein, creatinine, ions, conductivity) were determined using spectrophotometry and electrochemistry. All results were subjected to statistical processing for revealing different correlations between the studied parameters. These metabolites were observed in the urine obtained from healthy subjects and influence of storage conditions (freezing and thawing) on the concentration of addition of sarcosine was monitored.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Metallomics and Nanotechnologies

ISSN

2336-3940

e-ISSN

—

Svazek periodika

2

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

40-50

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—