Identification of Alleles of Puroindoline Genes and Their Effect on Wheat (Triticum aestivum L.) Grain Texture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43909019" target="_blank" >RIV/62156489:43210/16:43909019 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.ftb.com.hr/images/pdfarticles/2016/January-March/ftb-54-103.pdf" target="_blank" >http://www.ftb.com.hr/images/pdfarticles/2016/January-March/ftb-54-103.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17113/ftb.54.01.16.4119" target="_blank" >10.17113/ftb.54.01.16.4119</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of Alleles of Puroindoline Genes and Their Effect on Wheat (Triticum aestivum L.) Grain Texture

Popis výsledku v původním jazyce

Grain hardness is one of the most important quality characteristics of wheat (Triticum aestivum L.). It is a signifi cant property of wheat grains and relates to milling quality and end product quality. Grain hardness is caused by the presence of puroindoline genes (Pina and Pinb). A collection of 25 genotypes of wheat with unusual grain colour (blue aleurone, purple and white pericarp, yellow endosperm) was studied by polymerase chain reaction (PCR) for the diversity within Pina and Pinb (alleles: Pina-D1a, Pina-D1b, Pinb-D1a, Pinb--D1b, Pinb-D1c and Pinb-D1d). The endosperm structure was determined by a non-destructive method using light transfl ectance meter and grain hardness by a texture analyser. Genotype Novosibirskaya 67 and isogenic ANK lines revealed hitherto unknown alleles at the locus for the annealing of primers of Pinb-D1. Allele Pinb-D1c was found to be absent from each genotype. The mealy endosperm ranged from 0 to 100 % and grain hardness from 15.10 to 26.87 N per sample.

Název v anglickém jazyce

Identification of Alleles of Puroindoline Genes and Their Effect on Wheat (Triticum aestivum L.) Grain Texture

Popis výsledku anglicky

Grain hardness is one of the most important quality characteristics of wheat (Triticum aestivum L.). It is a signifi cant property of wheat grains and relates to milling quality and end product quality. Grain hardness is caused by the presence of puroindoline genes (Pina and Pinb). A collection of 25 genotypes of wheat with unusual grain colour (blue aleurone, purple and white pericarp, yellow endosperm) was studied by polymerase chain reaction (PCR) for the diversity within Pina and Pinb (alleles: Pina-D1a, Pina-D1b, Pinb-D1a, Pinb--D1b, Pinb-D1c and Pinb-D1d). The endosperm structure was determined by a non-destructive method using light transfl ectance meter and grain hardness by a texture analyser. Genotype Novosibirskaya 67 and isogenic ANK lines revealed hitherto unknown alleles at the locus for the annealing of primers of Pinb-D1. Allele Pinb-D1c was found to be absent from each genotype. The mealy endosperm ranged from 0 to 100 % and grain hardness from 15.10 to 26.87 N per sample.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Technology and Biotechnology

ISSN

1330-9862

e-ISSN

—

Svazek periodika

54

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

HR - Chorvatská republika

Počet stran výsledku

5

Strana od-do

103-107

Kód UT WoS článku

000374275500014

EID výsledku v databázi Scopus

2-s2.0-84964826081