Association between allelic variability at the Phytoene synthase 1 locus and yellow pigment content in wheat and tritordeum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25328859%3A_____%2F22%3AN0000063" target="_blank" >RIV/25328859:_____/22:N0000063 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Association between allelic variability at the Phytoene synthase 1 locus and yellow pigment content in wheat and tritordeum

Popis výsledku v původním jazyce

Endosperm carotenoid content in the cereals is a primary determinant of flour colour and this affects both the nutritional value of the grain and its utility for different applications. Utilising wheat rice synteny two genes, epsilon-cyclase (epsilon-LCY) and phytoene synthase (Psy-1), were identified as candidate genes for two of the QTL affecting carotenoid content in endosperm. Phytoene synthase (Psy-1) is a key gene that plays a main role in the biosynthetic pathway of carotenoids and affects their accumulation in grains. The aim of the study was to verify this role in genotypes with different grain colours for breeding. Grains of 14 genotypes of hexaploid wheat (standard wheat varieties with red grain: Bohemia, Julie, Matchball; with blue aleurone: Skorpion, AF Oxana, V1-80-17; with purple pericarp: Rosso, AF Jumiko, PS Karkulka; with yellow endosperm: Bona Vita, V2-49-17, Citrus; with black grain: V1-63-17, AF Zora) and 3 genotypes of tritordeum (HT 135, HTC 1380, and JB1) were analysed. The grains were from the harvest of the year 2018, 2019 and 2020. Spectrophotometrically measured values (Bulda et al., 2008) of total carotenoid content ranged from 0.71 to 14.82 μg/g depending on the genotype and the year of harvest. The total carotenoid content in grains of tritordeum genotypes was higher than of wheat. Molecular markers YP7A, YP7A-2, YP7B-1, YP7B2, YP7B4, YP7D-2 (Stepanenko et al., 2017), Psy1-A1_STS (Singh et al., 2009), PSY7A-1, PSY7A5, and PSY7A-7 (Crawford et al., 2011) have shown useful for analysis of variability of Psy-1 locus. 8 alleles of loci Psy-A1 (alleles a, b, c, l, and o), Psy-B1a or b, and Psy-D1a were detected in the total. There was statistically significant correlation (p<0.05) between carotenoids content and alleles combination in the analysed samples. The importance of studied gene on the content of carotenoids in grain and for the breeding program was verified. Mendel Genetics Conference. Book of Abstracts : A tribute to Gregor Johann Mendel on the bicentennial of his birth. 20-23 July, 2022 Brno, Czech Republic

Název v anglickém jazyce

Association between allelic variability at the Phytoene synthase 1 locus and yellow pigment content in wheat and tritordeum

Popis výsledku anglicky

Endosperm carotenoid content in the cereals is a primary determinant of flour colour and this affects both the nutritional value of the grain and its utility for different applications. Utilising wheat rice synteny two genes, epsilon-cyclase (epsilon-LCY) and phytoene synthase (Psy-1), were identified as candidate genes for two of the QTL affecting carotenoid content in endosperm. Phytoene synthase (Psy-1) is a key gene that plays a main role in the biosynthetic pathway of carotenoids and affects their accumulation in grains. The aim of the study was to verify this role in genotypes with different grain colours for breeding. Grains of 14 genotypes of hexaploid wheat (standard wheat varieties with red grain: Bohemia, Julie, Matchball; with blue aleurone: Skorpion, AF Oxana, V1-80-17; with purple pericarp: Rosso, AF Jumiko, PS Karkulka; with yellow endosperm: Bona Vita, V2-49-17, Citrus; with black grain: V1-63-17, AF Zora) and 3 genotypes of tritordeum (HT 135, HTC 1380, and JB1) were analysed. The grains were from the harvest of the year 2018, 2019 and 2020. Spectrophotometrically measured values (Bulda et al., 2008) of total carotenoid content ranged from 0.71 to 14.82 μg/g depending on the genotype and the year of harvest. The total carotenoid content in grains of tritordeum genotypes was higher than of wheat. Molecular markers YP7A, YP7A-2, YP7B-1, YP7B2, YP7B4, YP7D-2 (Stepanenko et al., 2017), Psy1-A1_STS (Singh et al., 2009), PSY7A-1, PSY7A5, and PSY7A-7 (Crawford et al., 2011) have shown useful for analysis of variability of Psy-1 locus. 8 alleles of loci Psy-A1 (alleles a, b, c, l, and o), Psy-B1a or b, and Psy-D1a were detected in the total. There was statistically significant correlation (p<0.05) between carotenoids content and alleles combination in the analysed samples. The importance of studied gene on the content of carotenoids in grain and for the breeding program was verified. Mendel Genetics Conference. Book of Abstracts : A tribute to Gregor Johann Mendel on the bicentennial of his birth. 20-23 July, 2022 Brno, Czech Republic

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910343" target="_blank" >QK1910343: Nové znaky pšenice pro zvýšení adaptačních možností v prostředí globální změny klimatu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů