Efficiency of Tetrameric Short Tandem Repeats in Prestice Black-Pied Pig for Traceability and Parentity Testing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43909136" target="_blank" >RIV/62156489:43210/16:43909136 - isvavai.cz</a>

Výsledek na webu

<a href="http://acta.mendelu.cz/media/pdf/actaun_2016064020557.pdf" target="_blank" >http://acta.mendelu.cz/media/pdf/actaun_2016064020557.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.11118/actaun201664020557" target="_blank" >10.11118/actaun201664020557</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Efficiency of Tetrameric Short Tandem Repeats in Prestice Black-Pied Pig for Traceability and Parentity Testing

Popis výsledku v původním jazyce

The main goal of the research was to evaluate suitability of 11 tetrameric STRs (Short Tandem Repeats) marker panel for the Prestice Black-Pied pig (PC) breed as the only genetic resource in the Czech Republic.The analysis was carried out in 522 breeding and slaughter PC individuals. We observed 94 alleles overall across whole 11 STR panel. The observed heterozygosity HO was 0.677, the polymorphism information content (PIC) was in average 0.664 per locus. The probability of identity of two independent samples (PI) using all 11 STR loci was 4.037.10 na -11 and the probability of identity related individuals (PISibs) was 8.315.10 na -5. The power of exclusion for loci combinations when both parents are known (P1), when only one of the parent is known (P2) and for two putative parents (P3) were 0.9996, 0.9899 and 0.9999. The efficiency of the 11 tetrameric STRs (Animaltype Pig kit) is higher in PC in comparison to commercial breeds and slaughter crossbred pigs. In genetic resource PC, the 11 STRs panel is usable for forensic purpose such parentity testing and traceability.

Název v anglickém jazyce

Efficiency of Tetrameric Short Tandem Repeats in Prestice Black-Pied Pig for Traceability and Parentity Testing

Popis výsledku anglicky

The main goal of the research was to evaluate suitability of 11 tetrameric STRs (Short Tandem Repeats) marker panel for the Prestice Black-Pied pig (PC) breed as the only genetic resource in the Czech Republic.The analysis was carried out in 522 breeding and slaughter PC individuals. We observed 94 alleles overall across whole 11 STR panel. The observed heterozygosity HO was 0.677, the polymorphism information content (PIC) was in average 0.664 per locus. The probability of identity of two independent samples (PI) using all 11 STR loci was 4.037.10 na -11 and the probability of identity related individuals (PISibs) was 8.315.10 na -5. The power of exclusion for loci combinations when both parents are known (P1), when only one of the parent is known (P2) and for two putative parents (P3) were 0.9996, 0.9899 and 0.9999. The efficiency of the 11 tetrameric STRs (Animaltype Pig kit) is higher in PC in comparison to commercial breeds and slaughter crossbred pigs. In genetic resource PC, the 11 STRs panel is usable for forensic purpose such parentity testing and traceability.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1210253" target="_blank" >QJ1210253: Využití metod molekulární genetiky jako nástroje pro efektivní plemenářskou práci v malé populaci prasat.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis

ISSN

1211-8516

e-ISSN

—

Svazek periodika

64

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

557-565

Kód UT WoS článku

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EID výsledku v databázi Scopus

2-s2.0-84970002326