Parameters of variability and diversity of tetrameric STRs for practical use in Zlotnicka White and Zlotnicka Spotted pigs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43911841" target="_blank" >RIV/62156489:43210/17:43911841 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/17:00113206

Výsledek na webu

<a href="http://www.ighz.edu.pl/uploaded/FSiBundleContentBlockBundleEntityTranslatableBlockTranslatableFilesElement/filePath/873/str253-264.pdf" target="_blank" >http://www.ighz.edu.pl/uploaded/FSiBundleContentBlockBundleEntityTranslatableBlockTranslatableFilesElement/filePath/873/str253-264.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Parameters of variability and diversity of tetrameric STRs for practical use in Zlotnicka White and Zlotnicka Spotted pigs

Popis výsledku v původním jazyce

As a goal of this paper, the assessment of genetic variability of Zlotnicka White (ZW) and Zlotnicka Spotted (ZS), was chosen in order to verify the appropriateness of 11 tetrameric Short Tandem Repeats (STRs) panel for use in genetic resources of pigs. Analyses were carried out in sets of 91 ZW and 250 ZS pigs. Seventy-one alleles in ZW and 85 alleles in ZS were detected at all 11 STRs loci. An average number of alleles at locus (MNA) was 6.455 in ZW and 7.727 in ZS. An average number of effective alleles (MNe) was 3.532 in ZW and 3.431 in ZS. Observed heterozygosity Ho was 0.659 in ZW and 0.637 in ZS. On average, polymorphism information content (PIC) reached 0.639 and 0.619 per locus in ZW and ZS. The probability of identity of two independent samples PI using all 11 STRs loci in ZW amounted to 3.118 x 10-10 and to 5.921 x 10-10 in ZS while the probability of identity related individuals PISibs was 1.331 x 10-4 and 1.749 x 10-4 in ZW and ZS. The power of exclusion for loci combinations when both parents are known, when only one of the parent is known and for two putative parents P1, P2 and P3 were in ZW versus ZS 0.99903 v. 0.99887, 0.97998 v. 0.97654 and 0.99999 v. 0.99998, respectively. Identified estimates of stated parameters illustrate suitability of tetrameric STRs for practical application in the management of genetic resources, verification of parentage and traceability in ZW and ZS. Based on the results, we recommend the panel of tetrameric STRs loci as suitable for parentage, traceability and differentiation of subpopulations in genetic pig resources of similar history. He.

Název v anglickém jazyce

Parameters of variability and diversity of tetrameric STRs for practical use in Zlotnicka White and Zlotnicka Spotted pigs

Popis výsledku anglicky

As a goal of this paper, the assessment of genetic variability of Zlotnicka White (ZW) and Zlotnicka Spotted (ZS), was chosen in order to verify the appropriateness of 11 tetrameric Short Tandem Repeats (STRs) panel for use in genetic resources of pigs. Analyses were carried out in sets of 91 ZW and 250 ZS pigs. Seventy-one alleles in ZW and 85 alleles in ZS were detected at all 11 STRs loci. An average number of alleles at locus (MNA) was 6.455 in ZW and 7.727 in ZS. An average number of effective alleles (MNe) was 3.532 in ZW and 3.431 in ZS. Observed heterozygosity Ho was 0.659 in ZW and 0.637 in ZS. On average, polymorphism information content (PIC) reached 0.639 and 0.619 per locus in ZW and ZS. The probability of identity of two independent samples PI using all 11 STRs loci in ZW amounted to 3.118 x 10-10 and to 5.921 x 10-10 in ZS while the probability of identity related individuals PISibs was 1.331 x 10-4 and 1.749 x 10-4 in ZW and ZS. The power of exclusion for loci combinations when both parents are known, when only one of the parent is known and for two putative parents P1, P2 and P3 were in ZW versus ZS 0.99903 v. 0.99887, 0.97998 v. 0.97654 and 0.99999 v. 0.99998, respectively. Identified estimates of stated parameters illustrate suitability of tetrameric STRs for practical application in the management of genetic resources, verification of parentage and traceability in ZW and ZS. Based on the results, we recommend the panel of tetrameric STRs loci as suitable for parentage, traceability and differentiation of subpopulations in genetic pig resources of similar history. He.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QJ1210253" target="_blank" >QJ1210253: Využití metod molekulární genetiky jako nástroje pro efektivní plemenářskou práci v malé populaci prasat.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal Science Papers and Reports

ISSN

0860-4037

e-ISSN

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Svazek periodika

35

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

11

Strana od-do

253-263

Kód UT WoS článku

000410770400004

EID výsledku v databázi Scopus

2-s2.0-85029142029