Protein metabolism disruption and its effect on plant growth

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43911092" target="_blank" >RIV/62156489:43210/16:43911092 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Protein metabolism disruption and its effect on plant growth

Popis výsledku v původním jazyce

More than 6 % of the Arabidopsis genome encodes components of the ubiquitin conjugation system and, in effect, most plant regulatory circuitry, and many steps of process execution, depend on ubiquitin modification. Recent years have shown that plant hormones operate through circuits in which targeted, conditional turnover of regulator proteins via proteasome are of critical importance. Thus, a disruption of the ubiquitin-proteasome system (UPS) would have a significant impact on plant growth and development. Here we employed a potent proteasome inhibitor and a transgenic Arabidopsis line RV 86-5 carrying modified inducible ubiquitin that prevents protein degradation via the UPS. We followed the effects on plant physiology and analyzed proteome-wide responses via an LC-MS. The results present evidence that an intricate balance between the UPS and cytokinin modulates the growth response output of cytokinin signaling. On the proteome level, an LC-MS profiling identified >4,000 proteins, 61 of which were previously identified in response to proteasome inhibition.

Název v anglickém jazyce

Protein metabolism disruption and its effect on plant growth

Popis výsledku anglicky

More than 6 % of the Arabidopsis genome encodes components of the ubiquitin conjugation system and, in effect, most plant regulatory circuitry, and many steps of process execution, depend on ubiquitin modification. Recent years have shown that plant hormones operate through circuits in which targeted, conditional turnover of regulator proteins via proteasome are of critical importance. Thus, a disruption of the ubiquitin-proteasome system (UPS) would have a significant impact on plant growth and development. Here we employed a potent proteasome inhibitor and a transgenic Arabidopsis line RV 86-5 carrying modified inducible ubiquitin that prevents protein degradation via the UPS. We followed the effects on plant physiology and analyzed proteome-wide responses via an LC-MS. The results present evidence that an intricate balance between the UPS and cytokinin modulates the growth response output of cytokinin signaling. On the proteome level, an LC-MS profiling identified >4,000 proteins, 61 of which were previously identified in response to proteasome inhibition.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů