The role of ubiquitin-conjugating enzymes during seed germination

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914667" target="_blank" >RIV/62156489:43210/18:43914667 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

The role of ubiquitin-conjugating enzymes during seed germination

Popis výsledku v původním jazyce

Regulated protein turn-over is one of the key determining factors of successful plant growth and development. Not only protein synthesis but also targeted protein degradation was widely reported to be involved in multiple metabolic or regulatory processes in cells (e.g. removing of negative regulators in different signalling pathways, mobilization of storage compounds or degradation of misfolded proteins). Seed germination is a crucial phase of plants life which is characterized with highly intensive proteome dynamics, caused by degradation of stored proteins and de novo proteosynthesis. Here, we focused on the effect of proteasome degradation on seed germination and seedling establishment, using Arabidopsis thaliana ubc mutant lines. Further, we employed an LC-MS profiling to study this effect at proteome level. Our results indicate a significant overlap of candidate proteins between all tested ubc lines, even though the reported expression patterns of chosen UBCs are significantly different.

Název v anglickém jazyce

The role of ubiquitin-conjugating enzymes during seed germination

Popis výsledku anglicky

Regulated protein turn-over is one of the key determining factors of successful plant growth and development. Not only protein synthesis but also targeted protein degradation was widely reported to be involved in multiple metabolic or regulatory processes in cells (e.g. removing of negative regulators in different signalling pathways, mobilization of storage compounds or degradation of misfolded proteins). Seed germination is a crucial phase of plants life which is characterized with highly intensive proteome dynamics, caused by degradation of stored proteins and de novo proteosynthesis. Here, we focused on the effect of proteasome degradation on seed germination and seedling establishment, using Arabidopsis thaliana ubc mutant lines. Further, we employed an LC-MS profiling to study this effect at proteome level. Our results indicate a significant overlap of candidate proteins between all tested ubc lines, even though the reported expression patterns of chosen UBCs are significantly different.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/7AMB17AT027" target="_blank" >7AMB17AT027: Cílená degradace proteinů v odpovědi na rostlinný hormon cytokinin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2018: Proceedings of International PhD Students Conference

ISBN

978-80-7509-597-8

ISSN

—

e-ISSN

neuvedeno

Počet stran výsledku

5

Strana od-do

325-329

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

7. 11. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000462205300060