Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43911526" target="_blank" >RIV/62156489:43210/17:43911526 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/17:00097482

Výsledek na webu

<a href="https://doi.org/10.1016/j.algal.2017.04.026" target="_blank" >https://doi.org/10.1016/j.algal.2017.04.026</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2017.04.026" target="_blank" >10.1016/j.algal.2017.04.026</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess

Popis výsledku v původním jazyce

Impact of ascorbate biosynthetic inhibitor (lycorine, LYC) on short-term cadmium (Cd) action (1 h, 100 μM) was studied in the green alga Scenedesmus quadricauda with 1 h or 24 h of LYC co-application. LYC suppressed Cd-induced increase in ascorbic acid and enhanced oxidative symptoms (ROS fluorescence) by modulating activities of antioxidative enzymes after 24 h of addition (decrease in superoxide dismutase, catalase and ascorbate peroxidase activities). Prolonged (24 h) exposure to LYC also affected content of Cd and quantitative changes of thiols (glutathione and phytochelatin 2) while Cd itself revealed interesting responses in algae maintained in the buffer for various time: these data indicate that in the studies involving axenic algal cultures, transfer of cells from sterile to non-sterile conditions modulates metal uptake. Among aliphatic organic acids, α-ketoglutaric, lactic and malic acids were variously influenced by Cd or lycorine, suggesting shift in the respiration and fermentation. It is concluded that ascorbic acid is involved in the protection of cells against Cd-induced ROS over-accumulation and metabolic changes were more expressive in the samples with 24 h co-application of LYC though side impacts of LYC cannot be excluded. DESI-Orbitrap MS for the identification and visualization of lycorine was also successfully tested here for the first time.

Název v anglickém jazyce

Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess

Popis výsledku anglicky

Impact of ascorbate biosynthetic inhibitor (lycorine, LYC) on short-term cadmium (Cd) action (1 h, 100 μM) was studied in the green alga Scenedesmus quadricauda with 1 h or 24 h of LYC co-application. LYC suppressed Cd-induced increase in ascorbic acid and enhanced oxidative symptoms (ROS fluorescence) by modulating activities of antioxidative enzymes after 24 h of addition (decrease in superoxide dismutase, catalase and ascorbate peroxidase activities). Prolonged (24 h) exposure to LYC also affected content of Cd and quantitative changes of thiols (glutathione and phytochelatin 2) while Cd itself revealed interesting responses in algae maintained in the buffer for various time: these data indicate that in the studies involving axenic algal cultures, transfer of cells from sterile to non-sterile conditions modulates metal uptake. Among aliphatic organic acids, α-ketoglutaric, lactic and malic acids were variously influenced by Cd or lycorine, suggesting shift in the respiration and fermentation. It is concluded that ascorbic acid is involved in the protection of cells against Cd-induced ROS over-accumulation and metabolic changes were more expressive in the samples with 24 h co-application of LYC though side impacts of LYC cannot be excluded. DESI-Orbitrap MS for the identification and visualization of lycorine was also successfully tested here for the first time.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algal Research-Biology, Biomass and Biofuels

ISSN

2211-9264

e-ISSN

—

Svazek periodika

24

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

354-359

Kód UT WoS článku

000404864600038

EID výsledku v databázi Scopus

2-s2.0-85018255061