New option for decreasing of concentration limit of detection in electrophoresis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912627" target="_blank" >RIV/62156489:43210/17:43912627 - isvavai.cz</a>
Výsledek na webu
<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
New option for decreasing of concentration limit of detection in electrophoresis
Popis výsledku v původním jazyce
A hyphenated method consisting of transient electrokinetic dosing on-line coupled to isotachophoretic analysis was developed for the pre-concentration, pre-separation and analytical determination of a model substance - anionic herbicide glyphosate (gly) - in aqueous samples containing very low concentration of the analyte of interest. Various parameters were investigated in the framework of an optimisation study; the aim was to reach minimal concentration limit of detection decreasing in minimum time. The developed method consists of 2 phases. In the first one, a sample with addition of convenient buffer (dosing electrolyte) is electrokinetically dosed to the isotachophoretic column with proper leading electrolyte. During the dosing time, a moving-boundary electrophoresis zone of accumulated sample is created in the column and it is slowly moving through column. The accumulation of zone is proportional to the time and driving current and to the composition of dosing electrolyte. After some time of accumulation of the zone, dosing electrolyte is replaced with terminating one. Now, the regular isotachophoretic separation and analysis starts. The electrolyte composition and the dosing time were thoroughly optimized and 14 fold of accumulation was reached in 25 minutes in comparison to classical isotachophoretic analysis. The method is simple and applicable to all commercial isotachophoretic analysers.
Název v anglickém jazyce
New option for decreasing of concentration limit of detection in electrophoresis
Popis výsledku anglicky
A hyphenated method consisting of transient electrokinetic dosing on-line coupled to isotachophoretic analysis was developed for the pre-concentration, pre-separation and analytical determination of a model substance - anionic herbicide glyphosate (gly) - in aqueous samples containing very low concentration of the analyte of interest. Various parameters were investigated in the framework of an optimisation study; the aim was to reach minimal concentration limit of detection decreasing in minimum time. The developed method consists of 2 phases. In the first one, a sample with addition of convenient buffer (dosing electrolyte) is electrokinetically dosed to the isotachophoretic column with proper leading electrolyte. During the dosing time, a moving-boundary electrophoresis zone of accumulated sample is created in the column and it is slowly moving through column. The accumulation of zone is proportional to the time and driving current and to the composition of dosing electrolyte. After some time of accumulation of the zone, dosing electrolyte is replaced with terminating one. Now, the regular isotachophoretic separation and analysis starts. The electrolyte composition and the dosing time were thoroughly optimized and 14 fold of accumulation was reached in 25 minutes in comparison to classical isotachophoretic analysis. The method is simple and applicable to all commercial isotachophoretic analysers.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
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OECD FORD obor
10403 - Physical chemistry
Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
MendelNet 2017: Proceedings of International PhD Students Conference
ISBN
978-80-7509-529-9
ISSN
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e-ISSN
neuvedeno
Počet stran výsledku
5
Strana od-do
884-888
Název nakladatele
Mendelova univerzita v Brně
Místo vydání
Brno
Místo konání akce
Brno
Datum konání akce
8. 11. 2017
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000440194500158