Multifunctional pipeteing platform for molecular biology and biochemistry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912640" target="_blank" >RIV/62156489:43210/17:43912640 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Multifunctional pipeteing platform for molecular biology and biochemistry

Popis výsledku v původním jazyce

The classical hand pipetting approach can be partially substituted in several cases by sophisticated devices that are taking over the necessity of sample manipulation. The developed pipetting method can facilitate routine laboratory work, but the created method has to meet several criteria including - minimizing the possibility of contamination. In this work is briefly summarized four years experiences with the liquid handling system BioNex Nanodrop II. This device is able to pipette (using eight pipetting tips) small volumes in the range of 0.100 µl up to 500 µl into the three common microplate formats - MTP 96, MTP 384 and MTP 1536. The possibility of sample (bacteria, bacterial plasmids and DNA templates for PCR) cross-contamination was estimated in three experiments. The cross-contamination was not presented or it was detected at very low level. The main advantages - speed and accuracy - of the automatic pipetting are described in the last experiment where the enzyme activity was measured.

Název v anglickém jazyce

Multifunctional pipeteing platform for molecular biology and biochemistry

Popis výsledku anglicky

The classical hand pipetting approach can be partially substituted in several cases by sophisticated devices that are taking over the necessity of sample manipulation. The developed pipetting method can facilitate routine laboratory work, but the created method has to meet several criteria including - minimizing the possibility of contamination. In this work is briefly summarized four years experiences with the liquid handling system BioNex Nanodrop II. This device is able to pipette (using eight pipetting tips) small volumes in the range of 0.100 µl up to 500 µl into the three common microplate formats - MTP 96, MTP 384 and MTP 1536. The possibility of sample (bacteria, bacterial plasmids and DNA templates for PCR) cross-contamination was estimated in three experiments. The cross-contamination was not presented or it was detected at very low level. The main advantages - speed and accuracy - of the automatic pipetting are described in the last experiment where the enzyme activity was measured.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10609 - Biochemical research methods

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2017: Proceedings of International PhD Students Conference

ISBN

978-80-7509-529-9

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

6

Strana od-do

943-948

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

8. 11. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000440194500169