TNF-alpha can induce lymphocyte apoptosis during experimentally induced mastitis caused by lipopolysaccharide

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912840" target="_blank" >RIV/62156489:43210/17:43912840 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/17:PU139980

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

TNF-alpha can induce lymphocyte apoptosis during experimentally induced mastitis caused by lipopolysaccharide

Popis výsledku v původním jazyce

Lipopolysaccharide is a toxin released from the cell wall of Gram-negative bacteria. Infusion of lipopolysaccharide into the bovine mammary gland induces an inflammatory response [1]. Mastitis is accompanied by the production of inflammatory cytokines [2,3]. Tumor necrosis factor alpha (TNF-a) is pro-inflammatory cytokine produced mainly by macrophages and also by some of other cell types as neutrophils. The aim of this study was to analyse the effect of TNF-a on induction of lymphocyte apoptosis during inflammatory response induced by lipopolysaccharide. The experiments were carried out on the mammary glands of virgin heifers. TNF-a concentration was quantified by ELISA in four time points following stimulation of mammary glands by (24, 48, 72, 168 hours). Apoptosis of lymphocytes was analysed by flow cytometry following simultaneous staining with Annexin-V and propidium iodide in the same time points. There was found high correlation between TNF-a concentration and apoptosis of lymphocytes which suggests that this cytokine can induce lymphocyte apoptosis following influx of neutrophils caused by lipopolysaccharide.

Název v anglickém jazyce

TNF-alpha can induce lymphocyte apoptosis during experimentally induced mastitis caused by lipopolysaccharide

Popis výsledku anglicky

Lipopolysaccharide is a toxin released from the cell wall of Gram-negative bacteria. Infusion of lipopolysaccharide into the bovine mammary gland induces an inflammatory response [1]. Mastitis is accompanied by the production of inflammatory cytokines [2,3]. Tumor necrosis factor alpha (TNF-a) is pro-inflammatory cytokine produced mainly by macrophages and also by some of other cell types as neutrophils. The aim of this study was to analyse the effect of TNF-a on induction of lymphocyte apoptosis during inflammatory response induced by lipopolysaccharide. The experiments were carried out on the mammary glands of virgin heifers. TNF-a concentration was quantified by ELISA in four time points following stimulation of mammary glands by (24, 48, 72, 168 hours). Apoptosis of lymphocytes was analysed by flow cytometry following simultaneous staining with Annexin-V and propidium iodide in the same time points. There was found high correlation between TNF-a concentration and apoptosis of lymphocytes which suggests that this cytokine can induce lymphocyte apoptosis following influx of neutrophils caused by lipopolysaccharide.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů