TGF-beta 1 production during experimentally induced mastitis caused by lipopolysaccharide

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

TGF-beta 1 production during experimentally induced mastitis caused by lipopolysaccharide

Popis výsledku v původním jazyce

Lipopolysaccharide is an endotoxin released from the cell wall of Gram-negative bacteria. Infusion of lipopolysaccha- ride into the bovine mammary gland induces an inflamma-tory response [1]. Inflammation of the mammary gland is accompanied by the production of inflammatory cytokines [2,3]. Transforming growth factor beta 1 (TGF-b1) is produced in very high levels by platelets. Other types of cells which produce this cytokine are macrophages, lymphocytes, endothelial cells and fibroblasts. The aim of this study was to evaluate concentration of TGF-b1 in course of in vivo experimentally induced bovine mastitis by lipopolysaccha- ride. The experiments were carried out on the mammary glands of virgin heifers. TGF-b1 concentration was quan-tified by ELISA in four time points following stimulation of mammary glands by lipopolysaccharide (24, 48, 72, 168 hours). The maximum of TGF-b1 concentration was measured 48 hours following intramammary injection of lipopolysaccharide. It seems to be that 48 hours is the time to start regeneration of damaged tissues because TGF-b1is the cytokine responsible for wound healing.

Název v anglickém jazyce

TGF-beta 1 production during experimentally induced mastitis caused by lipopolysaccharide

Popis výsledku anglicky

Lipopolysaccharide is an endotoxin released from the cell wall of Gram-negative bacteria. Infusion of lipopolysaccha- ride into the bovine mammary gland induces an inflamma-tory response [1]. Inflammation of the mammary gland is accompanied by the production of inflammatory cytokines [2,3]. Transforming growth factor beta 1 (TGF-b1) is produced in very high levels by platelets. Other types of cells which produce this cytokine are macrophages, lymphocytes, endothelial cells and fibroblasts. The aim of this study was to evaluate concentration of TGF-b1 in course of in vivo experimentally induced bovine mastitis by lipopolysaccha- ride. The experiments were carried out on the mammary glands of virgin heifers. TGF-b1 concentration was quan-tified by ELISA in four time points following stimulation of mammary glands by lipopolysaccharide (24, 48, 72, 168 hours). The maximum of TGF-b1 concentration was measured 48 hours following intramammary injection of lipopolysaccharide. It seems to be that 48 hours is the time to start regeneration of damaged tissues because TGF-b1is the cytokine responsible for wound healing.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů