Production of Interferon Gamma and Transforming Growth Factor Beta 1 from Bovine Mammary Gland Leukocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43909255" target="_blank" >RIV/62156489:43210/17:43909255 - isvavai.cz</a>

Výsledek na webu

<a href="http://docsdrive.com/pdfs/medwelljournals/javaa/2017/119-121.pdf" target="_blank" >http://docsdrive.com/pdfs/medwelljournals/javaa/2017/119-121.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Production of Interferon Gamma and Transforming Growth Factor Beta 1 from Bovine Mammary Gland Leukocytes

Popis výsledku v původním jazyce

The objective of this trial was to study the inflammatory cytokines interferon gamma (IFN-γ) and transforming growth factor beta 1 (TGF-β1) which are produced by bovine mammary gland leukocytes. The leukocytes were obtained by mammary gland lavage and incubated for 1, 2 and 18 hours with lipopolysaccharide (50 µg/ml) or with muramyl dipeptide (500 µg/ml) in vitro. Lipopolyccharide was used as a toxin of Gram-negative bacteria, and muramyl dipeptide as a structural unit of a peptidoglycan of Gram-positive bacteria. The concentration levels of these cytokines were analyzed by ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. Higher IFN-γ concentration was recorded for lipopolysacharide incubation. The production of IFN-γ started 18 hours following the cell incubation with lipopolysaccharide or with muramyl dipeptide. The production of TGF-β1 was higher for lipopolysaccharide incubation than for muramyl dipeptide incubation throughout the all set time points. The TGF-β1 production started 2 hours following the cell incubation with muramyl dipeptide.

Název v anglickém jazyce

Production of Interferon Gamma and Transforming Growth Factor Beta 1 from Bovine Mammary Gland Leukocytes

Popis výsledku anglicky

The objective of this trial was to study the inflammatory cytokines interferon gamma (IFN-γ) and transforming growth factor beta 1 (TGF-β1) which are produced by bovine mammary gland leukocytes. The leukocytes were obtained by mammary gland lavage and incubated for 1, 2 and 18 hours with lipopolysaccharide (50 µg/ml) or with muramyl dipeptide (500 µg/ml) in vitro. Lipopolyccharide was used as a toxin of Gram-negative bacteria, and muramyl dipeptide as a structural unit of a peptidoglycan of Gram-positive bacteria. The concentration levels of these cytokines were analyzed by ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. Higher IFN-γ concentration was recorded for lipopolysacharide incubation. The production of IFN-γ started 18 hours following the cell incubation with lipopolysaccharide or with muramyl dipeptide. The production of TGF-β1 was higher for lipopolysaccharide incubation than for muramyl dipeptide incubation throughout the all set time points. The TGF-β1 production started 2 hours following the cell incubation with muramyl dipeptide.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Animal and Veterinary Advances

ISSN

1680-5593

e-ISSN

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Svazek periodika

16

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

PK - Pákistánská islámská republika

Počet stran výsledku

3

Strana od-do

119-121

Kód UT WoS článku

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EID výsledku v databázi Scopus

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