The effect of peptidoglycan on production of pro-inflammatory cytokines by mammary gland leukocytes during in vitro study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914682" target="_blank" >RIV/62156489:43210/18:43914682 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of peptidoglycan on production of pro-inflammatory cytokines by mammary gland leukocytes during in vitro study

Popis výsledku v původním jazyce

Cytokine network is very complicated and new knowledge about these proteins is important to develop better system of prevention, diagnosis and therapy of mastitis including decrease of antibiotic treatment. The study was implemented on eight selected healthy heifers. Leukocytes were obtained by lavage of the mammary gland 24 hours following the mammary gland stimulation by phosphate buffered saline solution (PBS). Leukocytes were cultivated for three different times. The first were cultivated for one hour, next two hours and eighteen hours. For cultivating was using peptidoglycan. Concentration of IL-1β and IFN-γ was measured by ELISA. IL-1β has the higher concentration after one hour cultivation and IFN-γ after two hours cultivation. Stimulation of mammary gland leukocytes with peptidoglycan resulted in a significant increase in the concentration of cytokines in comparison to the control. The concentration of IL-1β peaked at 1-hour incubation. In 2 and 18 hours, there were found decreasing of the concentration of this cytokine.

Název v anglickém jazyce

The effect of peptidoglycan on production of pro-inflammatory cytokines by mammary gland leukocytes during in vitro study

Popis výsledku anglicky

Cytokine network is very complicated and new knowledge about these proteins is important to develop better system of prevention, diagnosis and therapy of mastitis including decrease of antibiotic treatment. The study was implemented on eight selected healthy heifers. Leukocytes were obtained by lavage of the mammary gland 24 hours following the mammary gland stimulation by phosphate buffered saline solution (PBS). Leukocytes were cultivated for three different times. The first were cultivated for one hour, next two hours and eighteen hours. For cultivating was using peptidoglycan. Concentration of IL-1β and IFN-γ was measured by ELISA. IL-1β has the higher concentration after one hour cultivation and IFN-γ after two hours cultivation. Stimulation of mammary gland leukocytes with peptidoglycan resulted in a significant increase in the concentration of cytokines in comparison to the control. The concentration of IL-1β peaked at 1-hour incubation. In 2 and 18 hours, there were found decreasing of the concentration of this cytokine.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2018: Proceedings of International PhD Students Conference

ISBN

978-80-7509-597-8

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

4

Strana od-do

388-391

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

7. 11. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000462205300073