Production of Cytokines from Bovine Mammary Gland Leukocytes Influenced by Bacterial Infection

Kód výsledku v IS VaVaI

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Výsledek na webu

<a href="https://sites.google.com/site/physiologyanimal2016/home/prispevky" target="_blank" >https://sites.google.com/site/physiologyanimal2016/home/prispevky</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Production of Cytokines from Bovine Mammary Gland Leukocytes Influenced by Bacterial Infection

Popis výsledku v původním jazyce

The objective of this trial was to study the inflammatory cytokines, which are produced by bovine mammary gland leukocytes. The leukocytes were obtained by mammary gland lavage and incubated for 1, 2 and 18 hours with lipopolysaccharide (50 μg/ml) or with muramyldipeptide (500 μg/ml) in vitro. LPS was used as a toxin of Gram-negative bacteria, and muramyldipeptide as a structural unit of a peptidoglycan of Gram-positive bacteria. The concentration levels of these cytokines were analyzed by ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. The IL-1β concentration level was higher for lipopolysacharide incubation than for muramyldipeptide incubation. The production of IL- 1β started 2 hours following the cell incubation with lipopolysacharide. The production of IL- 1β started 18 hours following the cell incubation with muramyldipeptide. The production of IL-4 started 1, 2, and 18 hours following the cell incubation with both bacteria components. Higher IL-4 concentration was recorded for lipopolysacharide incubation.

Název v anglickém jazyce

Production of Cytokines from Bovine Mammary Gland Leukocytes Influenced by Bacterial Infection

Popis výsledku anglicky

The objective of this trial was to study the inflammatory cytokines, which are produced by bovine mammary gland leukocytes. The leukocytes were obtained by mammary gland lavage and incubated for 1, 2 and 18 hours with lipopolysaccharide (50 μg/ml) or with muramyldipeptide (500 μg/ml) in vitro. LPS was used as a toxin of Gram-negative bacteria, and muramyldipeptide as a structural unit of a peptidoglycan of Gram-positive bacteria. The concentration levels of these cytokines were analyzed by ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. The IL-1β concentration level was higher for lipopolysacharide incubation than for muramyldipeptide incubation. The production of IL- 1β started 2 hours following the cell incubation with lipopolysacharide. The production of IL- 1β started 18 hours following the cell incubation with muramyldipeptide. The production of IL-4 started 1, 2, and 18 hours following the cell incubation with both bacteria components. Higher IL-4 concentration was recorded for lipopolysacharide incubation.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

30102 - Immunology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Animal Physiology 2016: Conference Proceedings

ISBN

978-80-7509-416-2

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

93-98

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Bořetice

Datum konání akce

13. 6. 2016

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000400583400015