Inflammatory cytokines produced by leukocytes of bovine mammary gland
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912601" target="_blank" >RIV/62156489:43210/17:43912601 - isvavai.cz</a>
Výsledek na webu
<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Inflammatory cytokines produced by leukocytes of bovine mammary gland
Popis výsledku v původním jazyce
The aim of this work was to study inflammatory cytokines - the tumor necrosis factor alpha (TNF-α) and the interleukin 10 (IL-10), which are produced by bovine mammary gland leukocytes. Eight clinically healthy heifers were selected for this study. Obtained leukocytes were incubated for 1, 2 and 18 hours with lipopolysaccharide (LPS) or muramyldipeptide (MDP) under in vitro conditions. Concentration of TNF-α and IL-10 was measured by ELISA method. Production of TNF-α by leukocytes of bovine mammary gland was higher (LPS:552.57 pg/ml [+-27.35]; MDP:680.20 pg/ml [+-31.32]) than production of TNF-α by blood leukocytes (LPS:344.87 pg/ml [+-23.12]; MDP:285.56 pg/ml [+-19.87]). Concentration of TNF-α was increased after 18 hours of incubation. IL-10 production by bovine mammary gland leukocytes was higher (LPS:26.02 u/ml [+-3.44] and 36.98 u/ml [+-7.78]; MDP:22.33 u/ml [+-3.02] and 33.45 u/ml [+-8.28]) than production of IL-10 by blood leukocytes (LPS:12.67 u/ml [+-2.47]; MDP:9.45 u/ml [+-3.61]). IL-10 concentration of bovine mammary gland leukocytes was increased after 2 hours of incubation. Differential blood leukocyte count was determined by method of light microscopy.
Název v anglickém jazyce
Inflammatory cytokines produced by leukocytes of bovine mammary gland
Popis výsledku anglicky
The aim of this work was to study inflammatory cytokines - the tumor necrosis factor alpha (TNF-α) and the interleukin 10 (IL-10), which are produced by bovine mammary gland leukocytes. Eight clinically healthy heifers were selected for this study. Obtained leukocytes were incubated for 1, 2 and 18 hours with lipopolysaccharide (LPS) or muramyldipeptide (MDP) under in vitro conditions. Concentration of TNF-α and IL-10 was measured by ELISA method. Production of TNF-α by leukocytes of bovine mammary gland was higher (LPS:552.57 pg/ml [+-27.35]; MDP:680.20 pg/ml [+-31.32]) than production of TNF-α by blood leukocytes (LPS:344.87 pg/ml [+-23.12]; MDP:285.56 pg/ml [+-19.87]). Concentration of TNF-α was increased after 18 hours of incubation. IL-10 production by bovine mammary gland leukocytes was higher (LPS:26.02 u/ml [+-3.44] and 36.98 u/ml [+-7.78]; MDP:22.33 u/ml [+-3.02] and 33.45 u/ml [+-8.28]) than production of IL-10 by blood leukocytes (LPS:12.67 u/ml [+-2.47]; MDP:9.45 u/ml [+-3.61]). IL-10 concentration of bovine mammary gland leukocytes was increased after 2 hours of incubation. Differential blood leukocyte count was determined by method of light microscopy.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
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OECD FORD obor
40301 - Veterinary science
Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
MendelNet 2017: Proceedings of International PhD Students Conference
ISBN
978-80-7509-529-9
ISSN
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e-ISSN
neuvedeno
Počet stran výsledku
5
Strana od-do
708-712
Název nakladatele
Mendelova univerzita v Brně
Místo vydání
Brno
Místo konání akce
Brno
Datum konání akce
8. 11. 2017
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000440194500126