TNF-α and IL-10 are produced by leukocytes during the experimental inflammatory response of bovine mammary gland induced by peptidoglycan

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914679" target="_blank" >RIV/62156489:43210/18:43914679 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

TNF-α and IL-10 are produced by leukocytes during the experimental inflammatory response of bovine mammary gland induced by peptidoglycan

Popis výsledku v původním jazyce

The aim of this work was to analyse inflammatory cytokines - the tumour necrosis factor alpha (TNF-α) and the interleukin 10 (IL-10) during an inflammatory response of bovine mammary gland induced by peptidoglycan. The study was carried out on clinically healthy heifers. Obtained leukocytes were incubated for 24, 48, 72 and 168 hours with phosphate buffered saline (PBS) or with peptidoglycan under in vivo conditions. The concentration of TNF-α by leukocytes of the bovine mammary gland was higher [PBS: 147.82 pg/ml (+-22.56), peptidoglycan: 822.43 pg/ml (+-80.07)] than the production of IL-10 by bovine mammary gland leukocytes [PBS: 0 pg/ml (+-0), peptidoglycan: 35.56 pg/ml (+-7.88)]. Level of IL-10 was the highest in 72 hours of incubation in the mammary gland. Level of TNF-α was the highest in 24 hours of incubation in the mammary gland.

Název v anglickém jazyce

TNF-α and IL-10 are produced by leukocytes during the experimental inflammatory response of bovine mammary gland induced by peptidoglycan

Popis výsledku anglicky

The aim of this work was to analyse inflammatory cytokines - the tumour necrosis factor alpha (TNF-α) and the interleukin 10 (IL-10) during an inflammatory response of bovine mammary gland induced by peptidoglycan. The study was carried out on clinically healthy heifers. Obtained leukocytes were incubated for 24, 48, 72 and 168 hours with phosphate buffered saline (PBS) or with peptidoglycan under in vivo conditions. The concentration of TNF-α by leukocytes of the bovine mammary gland was higher [PBS: 147.82 pg/ml (+-22.56), peptidoglycan: 822.43 pg/ml (+-80.07)] than the production of IL-10 by bovine mammary gland leukocytes [PBS: 0 pg/ml (+-0), peptidoglycan: 35.56 pg/ml (+-7.88)]. Level of IL-10 was the highest in 72 hours of incubation in the mammary gland. Level of TNF-α was the highest in 24 hours of incubation in the mammary gland.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2018: Proceedings of International PhD Students Conference

ISBN

978-80-7509-597-8

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

4

Strana od-do

376-379

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

7. 11. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000462205300070