Effect of Lipopolysaccharide and Muramyl Dipeptide on the Inflammatory Cytokine Production throughout Inflammatory Response of Bovine Mammary Gland

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43909254" target="_blank" >RIV/62156489:43210/18:43909254 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.medwelljournals.com/abstract/?doi=javaa.2018.100.103" target="_blank" >https://www.medwelljournals.com/abstract/?doi=javaa.2018.100.103</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of Lipopolysaccharide and Muramyl Dipeptide on the Inflammatory Cytokine Production throughout Inflammatory Response of Bovine Mammary Gland

Popis výsledku v původním jazyce

The objective of this study was to get a better knowledge of inflammatory cytokine production (TNF-α, IL-4, IL-10, and TGFβ1) throughout inflammatory response of bovine mammary gland caused by bacterial toxin lipopolysacharide (LPS) and muramyl dipeptide as a structural unit of peptidoglycan (MDP). In the experiments, there were used LPS and MDP at the specified concentrations (5 µg and 500 µg, respectively). The inflammatory response was analyzed at selected time points (24, 48, 72, and 168 h) following stimulation of the mammary glands. As a control, the same volumes of physiological solution was used. The mammary gland lavages were collected from the 8 clinically healthy virgin Holstein x Czech Pied heifers and the concentrations of the cytokines were analyzed by an ELISA method.

Název v anglickém jazyce

Effect of Lipopolysaccharide and Muramyl Dipeptide on the Inflammatory Cytokine Production throughout Inflammatory Response of Bovine Mammary Gland

Popis výsledku anglicky

The objective of this study was to get a better knowledge of inflammatory cytokine production (TNF-α, IL-4, IL-10, and TGFβ1) throughout inflammatory response of bovine mammary gland caused by bacterial toxin lipopolysacharide (LPS) and muramyl dipeptide as a structural unit of peptidoglycan (MDP). In the experiments, there were used LPS and MDP at the specified concentrations (5 µg and 500 µg, respectively). The inflammatory response was analyzed at selected time points (24, 48, 72, and 168 h) following stimulation of the mammary glands. As a control, the same volumes of physiological solution was used. The mammary gland lavages were collected from the 8 clinically healthy virgin Holstein x Czech Pied heifers and the concentrations of the cytokines were analyzed by an ELISA method.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Animal and Veterinary Advances

ISSN

1680-5593

e-ISSN

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Svazek periodika

17

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

PK - Pákistánská islámská republika

Počet stran výsledku

4

Strana od-do

100-103

Kód UT WoS článku

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EID výsledku v databázi Scopus

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