Production of interleukin 1beta and interferon gamma by bovine mammary gland leukocytes stimulated by lipopolysaccharide

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914684" target="_blank" >RIV/62156489:43210/18:43914684 - isvavai.cz</a>

Výsledek na webu

<a href="https://dx.doi.org/10.15414/jmbfs.2018-19.8.3.882-884" target="_blank" >https://dx.doi.org/10.15414/jmbfs.2018-19.8.3.882-884</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15414/jmbfs.2018-19.8.3.882-884" target="_blank" >10.15414/jmbfs.2018-19.8.3.882-884</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Production of interleukin 1beta and interferon gamma by bovine mammary gland leukocytes stimulated by lipopolysaccharide

Popis výsledku v původním jazyce

The aim of this trial was to investigate the production of the pro-inflammatory cytokines interleukin 1beta (IL-1β) and interferon gamma (IFN-γ) produced by bovine mammary gland leukocytes following lipopolysaccharide (LPS) challenge (5 μg LPS of Escherichia coliin 20 ml of PBS) or PBS (20 ml) as a control. The leukocytes were obtained by mammary gland lavage 1, 2, 3, and 7 days following the stimulation. The concentration levels of these cytokines were analyzed by sandwich ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. Higher average number of leukocytes (P&lt;0.01) was recorded for LPS than for PBS stimulation on day 1. The production of IL-1β increased following the stimulation on day 1. After that gradual decline was detected. The IFN-γ production showed a similar trend. Positive correlation between IL-1β and IFN-γ increased level was found.

Název v anglickém jazyce

Production of interleukin 1beta and interferon gamma by bovine mammary gland leukocytes stimulated by lipopolysaccharide

Popis výsledku anglicky

The aim of this trial was to investigate the production of the pro-inflammatory cytokines interleukin 1beta (IL-1β) and interferon gamma (IFN-γ) produced by bovine mammary gland leukocytes following lipopolysaccharide (LPS) challenge (5 μg LPS of Escherichia coliin 20 ml of PBS) or PBS (20 ml) as a control. The leukocytes were obtained by mammary gland lavage 1, 2, 3, and 7 days following the stimulation. The concentration levels of these cytokines were analyzed by sandwich ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. Higher average number of leukocytes (P&lt;0.01) was recorded for LPS than for PBS stimulation on day 1. The production of IL-1β increased following the stimulation on day 1. After that gradual decline was detected. The IFN-γ production showed a similar trend. Positive correlation between IL-1β and IFN-γ increased level was found.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiology, Biotechnology and Food Science

ISSN

1338-5178

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

3

Strana od-do

882-884

Kód UT WoS článku

000455221000005

EID výsledku v databázi Scopus

2-s2.0-85064629841