In-capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914200" target="_blank" >RIV/62156489:43210/18:43914200 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/18:PU132283

Výsledek na webu

<a href="https://doi.org/10.1002/jssc.201800591" target="_blank" >https://doi.org/10.1002/jssc.201800591</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201800591" target="_blank" >10.1002/jssc.201800591</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In-capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

Popis výsledku v původním jazyce

In-capillary derivatization using fluorescamine as the labeling reagent was proposed to enhance the detectability of adamantine drugs (memantine, amantadine and rimantadine) by spectrophotometric detection. Fluorescamine and the drugs were delivered to the capillary electrophoresis instrument at a ratio of 10:1 by zone injection. The derivatization reaction occurred following the application of voltage (20kV). The derivatized products, hydrolyzed- fluorescamine and excess fluorescamine were separated in 7min using 100mM borate buffer (pH10.0) containing 0.1% w/v of Brij (R)-35 and 20% v/v of acetonitrile. Validation data showed good linearity (r(2)&gt;0.98), precision (%RSDs&lt;3.4), and accuracy (recoveries ranging from 98.0 to 102.0%). The detection and quantitation limits are in the range of 6.0-8.5 and 18-25M, respectively. The validation data is comparable to reported methods, however, the current method offers better precision with enhanced sensitivity (up to six times). Applications of the method show percent labeled amounts found in the studied samples within 100.6-109.3%, which complied with the United States Pharmacopeia limit (90.0-110.0%). The method was simple, rapid and, automated, which required no extra instrumentation or skillful operators.

Název v anglickém jazyce

In-capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

Popis výsledku anglicky

In-capillary derivatization using fluorescamine as the labeling reagent was proposed to enhance the detectability of adamantine drugs (memantine, amantadine and rimantadine) by spectrophotometric detection. Fluorescamine and the drugs were delivered to the capillary electrophoresis instrument at a ratio of 10:1 by zone injection. The derivatization reaction occurred following the application of voltage (20kV). The derivatized products, hydrolyzed- fluorescamine and excess fluorescamine were separated in 7min using 100mM borate buffer (pH10.0) containing 0.1% w/v of Brij (R)-35 and 20% v/v of acetonitrile. Validation data showed good linearity (r(2)&gt;0.98), precision (%RSDs&lt;3.4), and accuracy (recoveries ranging from 98.0 to 102.0%). The detection and quantitation limits are in the range of 6.0-8.5 and 18-25M, respectively. The validation data is comparable to reported methods, however, the current method offers better precision with enhanced sensitivity (up to six times). Applications of the method show percent labeled amounts found in the studied samples within 100.6-109.3%, which complied with the United States Pharmacopeia limit (90.0-110.0%). The method was simple, rapid and, automated, which required no extra instrumentation or skillful operators.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Svazek periodika

41

Číslo periodika v rámci svazku

19

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

3764-3771

Kód UT WoS článku

000446667500012

EID výsledku v databázi Scopus

2-s2.0-85052483468