Chiral resolution of cationic piperazine derivatives by capillary electrophoresis using sulfated β-cyclodextrin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F24%3A73627498" target="_blank" >RIV/61989592:15310/24:73627498 - isvavai.cz</a>

Výsledek na webu

<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.202300271" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.202300271</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.202300271" target="_blank" >10.1002/elps.202300271</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chiral resolution of cationic piperazine derivatives by capillary electrophoresis using sulfated β-cyclodextrin

Popis výsledku v původním jazyce

This research focuses on the development and validation of a capillary electrophoresis (CE) method for the chiral separation of three H1-antihistamine drugs chlorcyclizine, norchlorcyclizine, and neobenodine using sulfated beta-cyclodextrin (S-beta-CD) as the chiral selector. The study explores various factors influencing the separation efficiency, including CD concentration, organic modifier content, voltage application, and buffer pH. Optimal conditions were identified as a 100 mM phosphate buffer (pH 6.0) with 34 mg mL-1 S-beta-CD and 40% (v/v) methanol. The method demonstrated excellent linearity in calibration curves, with coefficients of determination exceeding 0.99 for each enantiomer. Precision studies revealed good intra- and inter-day precision for migration times and peak areas. The limits of detection and quantification for the analytes were within the ranges of 5.9-11.4 and 18-34.6 mu mol L-1, respectively. Overall, the developed CE method offers a robust and precise approach for the chiral separation of H1-antihistamine drugs, holding promise for pharmaceutical applications.

Název v anglickém jazyce

Chiral resolution of cationic piperazine derivatives by capillary electrophoresis using sulfated β-cyclodextrin

Popis výsledku anglicky

This research focuses on the development and validation of a capillary electrophoresis (CE) method for the chiral separation of three H1-antihistamine drugs chlorcyclizine, norchlorcyclizine, and neobenodine using sulfated beta-cyclodextrin (S-beta-CD) as the chiral selector. The study explores various factors influencing the separation efficiency, including CD concentration, organic modifier content, voltage application, and buffer pH. Optimal conditions were identified as a 100 mM phosphate buffer (pH 6.0) with 34 mg mL-1 S-beta-CD and 40% (v/v) methanol. The method demonstrated excellent linearity in calibration curves, with coefficients of determination exceeding 0.99 for each enantiomer. Precision studies revealed good intra- and inter-day precision for migration times and peak areas. The limits of detection and quantification for the analytes were within the ranges of 5.9-11.4 and 18-34.6 mu mol L-1, respectively. Overall, the developed CE method offers a robust and precise approach for the chiral separation of H1-antihistamine drugs, holding promise for pharmaceutical applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA19-23033S" target="_blank" >GA19-23033S: Zlepšení citlivosti detekce v chirálních separacích pomocí kapilární elektroforézy ve spojení s ESI-MS nebo ICP-MS</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ELECTROPHORESIS

ISSN

0173-0835

e-ISSN

1522-2683

Svazek periodika

45

Číslo periodika v rámci svazku

17-18

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

1479-1486

Kód UT WoS článku

001197014500001

EID výsledku v databázi Scopus

2-s2.0-85190359986