Enantiomeric separation of R,S-tolterodine and R,S-methoxytolterodine with negatively charged cyclodextrins by capillary electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33147348" target="_blank" >RIV/61989592:15310/13:33147348 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jssc.201200530" target="_blank" >http://dx.doi.org/10.1002/jssc.201200530</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201200530" target="_blank" >10.1002/jssc.201200530</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enantiomeric separation of R,S-tolterodine and R,S-methoxytolterodine with negatively charged cyclodextrins by capillary electrophoresis

Popis výsledku v původním jazyce

The methods for separation of R,S-tolterodine and R,S-methoxytolterodine enantiomers using sulfated -, -CD and phosphated--CD by CE in acidic BGE based on Tris/phosphate pH 2.5 buffer were developed. Sulfated - and -CD allow anodic detection while phosphated--CD allows only cathodic detection of the separated enantiomers. The influence of chiral selector (CS)'s concentration as well as the influence of composition and concentration of BGE on resolutions were studied. Reversal migration order of tolterodine and methoxytolterodine enantiomers was observed, when sulfated-- and sulfated--CD were used. The developed methods with all three studied CSs, were validated and compared. All proposed methods enable determination of 0.2% of S-tolterodine as an optical impurity in pills, however the method with phosphated--CD provided lower detection limit, better repeatability of peak areas and migration times, and also lower consumption of CS. Developed method employing phosphated--CD that was appl

Název v anglickém jazyce

Enantiomeric separation of R,S-tolterodine and R,S-methoxytolterodine with negatively charged cyclodextrins by capillary electrophoresis

Popis výsledku anglicky

The methods for separation of R,S-tolterodine and R,S-methoxytolterodine enantiomers using sulfated -, -CD and phosphated--CD by CE in acidic BGE based on Tris/phosphate pH 2.5 buffer were developed. Sulfated - and -CD allow anodic detection while phosphated--CD allows only cathodic detection of the separated enantiomers. The influence of chiral selector (CS)'s concentration as well as the influence of composition and concentration of BGE on resolutions were studied. Reversal migration order of tolterodine and methoxytolterodine enantiomers was observed, when sulfated-- and sulfated--CD were used. The developed methods with all three studied CSs, were validated and compared. All proposed methods enable determination of 0.2% of S-tolterodine as an optical impurity in pills, however the method with phosphated--CD provided lower detection limit, better repeatability of peak areas and migration times, and also lower consumption of CS. Developed method employing phosphated--CD that was appl

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

9-10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

1561-1567

Kód UT WoS článku

000319866800009

EID výsledku v databázi Scopus

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