Determination of binding constants of multiple charged cyclodextrin complexes by ACE using uncorrected and ionic strength corrected actual mobilities of the species involved

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00517791" target="_blank" >RIV/61388963:_____/20:00517791 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201900352" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201900352</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201900352" target="_blank" >10.1002/elps.201900352</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of binding constants of multiple charged cyclodextrin complexes by ACE using uncorrected and ionic strength corrected actual mobilities of the species involved

Popis výsledku v původním jazyce

In this study, the apparent binding constants and limiting mobilities of the multiply charged complexes of the Δ− and Λ−enantiomers of Ru(II)‐ and Fe(II)‐polypyridyl associates ([Ru(2,2′‐bipyridine)3]2+, [Ru(1,10‐phenanthroline)3]2+, and [Fe(1,10‐phenanthroline)3]2+) with single‐isomer 2,3‐diacetylated‐6‐sulfated‐cyclodextrins (CDs) (12Ac‐6S‐α‐CD, 14Ac‐7S‐β‐CD, and 16Ac‐8S‐γ‐CD) were determined by ACE using uncorrected and ionic strength corrected actual mobilities of the species involved. Two limiting models were tested for the ionic strength correction of the actual mobilities based on an empirical relation for the ionic strength correction of multivalent ionic species. In model 1, the nominal values of the charge numbers (zS,nom) and analytical concentrations (cS,nom) of the above CD selectors in the BGEs were applied for calculation of the BGE ionic strength, as usual. In model 2, the CD selectors were considered as singly charged species (zS = −1) with |zS,nom|‐times higher concentrations in the BGE than their analytical concentrations (cS = |zS,nom| × cS,nom) in the calculation of the BGE ionic strength. In all three cases–with uncorrected actual mobilities as well as with actual mobilities corrected according to the two limiting models–the measured effective mobilities of the above enantiomers fit well the theoretical curves of their mobility dependences on the CD selectors concentrations in the BGE, with high average coefficients of determination (R2 = 0.9890–0.9995). Nevertheless, the best physico‐chemically meaningful values of the apparent binding constants and the limiting mobilities of the enantiomer‐CDs complexes with low RSDs were obtained using the actual mobilities of the species involved corrected according to model 2.

Název v anglickém jazyce

Determination of binding constants of multiple charged cyclodextrin complexes by ACE using uncorrected and ionic strength corrected actual mobilities of the species involved

Popis výsledku anglicky

In this study, the apparent binding constants and limiting mobilities of the multiply charged complexes of the Δ− and Λ−enantiomers of Ru(II)‐ and Fe(II)‐polypyridyl associates ([Ru(2,2′‐bipyridine)3]2+, [Ru(1,10‐phenanthroline)3]2+, and [Fe(1,10‐phenanthroline)3]2+) with single‐isomer 2,3‐diacetylated‐6‐sulfated‐cyclodextrins (CDs) (12Ac‐6S‐α‐CD, 14Ac‐7S‐β‐CD, and 16Ac‐8S‐γ‐CD) were determined by ACE using uncorrected and ionic strength corrected actual mobilities of the species involved. Two limiting models were tested for the ionic strength correction of the actual mobilities based on an empirical relation for the ionic strength correction of multivalent ionic species. In model 1, the nominal values of the charge numbers (zS,nom) and analytical concentrations (cS,nom) of the above CD selectors in the BGEs were applied for calculation of the BGE ionic strength, as usual. In model 2, the CD selectors were considered as singly charged species (zS = −1) with |zS,nom|‐times higher concentrations in the BGE than their analytical concentrations (cS = |zS,nom| × cS,nom) in the calculation of the BGE ionic strength. In all three cases–with uncorrected actual mobilities as well as with actual mobilities corrected according to the two limiting models–the measured effective mobilities of the above enantiomers fit well the theoretical curves of their mobility dependences on the CD selectors concentrations in the BGE, with high average coefficients of determination (R2 = 0.9890–0.9995). Nevertheless, the best physico‐chemically meaningful values of the apparent binding constants and the limiting mobilities of the enantiomer‐CDs complexes with low RSDs were obtained using the actual mobilities of the species involved corrected according to model 2.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Svazek periodika

41

Číslo periodika v rámci svazku

7/8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

13

Strana od-do

523-535

Kód UT WoS článku

000500308100001

EID výsledku v databázi Scopus

2-s2.0-85076163030